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          Institute: MPI für biophysikalische Chemie     Collection: Molekulare Entwicklungsbiologie (Prof. Herbert Jäckle)     Display Documents

ID: 17022.0, MPI für biophysikalische Chemie / Molekulare Entwicklungsbiologie (Prof. Herbert Jäckle)
Mitochondrial and cytoplasmic thioredoxin reductase variants encoded by a single Drosophila gene are both essential for viability
Authors:Missirlis, F.; Ulschmid, J. K.; Hirosawa-Takamori, M.; Groenke, S.; Schaefer, U.; Becker, K.; Phillips, J. P.; Jaeckle, H.
Date of Publication (YYYY-MM-DD):2002-03-29
Title of Journal:Journal of Biological Chemistry
Issue / Number:13
Start Page:11521
End Page:11526
Review Status:Peer-review
Audience:Not Specified
Abstract / Description:Defense against oxidative stress in mammals includes the regeneration of the major thiol reductants glutathione and thioredoxin by glutathione reductase and thioredoxin reductase (TrxR), respectively. In contrast, Drosophila, and possibly insects in general, lacks glutathione reductase and must rely solely on the TrxR system. The mammalian TrxRs described so far are selenoproteins that utilize NADPH to reduce protein as well as nonprotein substrates in mitochondria and cytoplasm of cells. We show that a single Drosophila gene, Trxr-1, encodes non-selenocysteine-containing cytoplasmic and mitochondrial TrxR isoforms that differ with respect to their N termini. We generated transcript-specific mutants and used in vivo approaches to explore the biological functions of the two enzyme variants by introducing the corresponding transgenes into different Trxr-1 mutants. The results show that, although the two TrxR isoforms have similar biochemical properties, their biological functions are not interchangeable.
Comment of the Author/Creator:Date: 2002, MAR 29
External Publication Status:published
Document Type:Article
Communicated by:N. N.
Affiliations:MPI für biophysikalische Chemie/Abt. Herbert Jäckle / 170
External Affiliations:Univ Giessen, Interdisziplinaires Forschungszentrum, D-35392 Giessen, Germany; Univ Guelph, Dept Mol Biol & Genet, Guelph, ON N1G 2W1, Canada
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