Please note that eDoc will be permanently shut down in the first quarter of 2021!      Home News About Us Contact Contributors Disclaimer Privacy Policy Help FAQ

Home
Search
Quick Search
Advanced
Fulltext
Browse
Collections
Persons
My eDoc
Session History
Login
Name:
Password:
Documentation
Help
Support Wiki
Direct access to
document ID:


          Institute: MPI für biophysikalische Chemie     Collection: NanoBiophotonik (Dr. Stefan Hell)     Display Documents



  history
ID: 17041.0, MPI für biophysikalische Chemie / NanoBiophotonik (Dr. Stefan Hell)
Comparison of the axial resolution of practical Nipkow-disk confocal fluorescence microscopy with that of multifocal multiphoton microscopy: theory and experiment
Authors:Egner, A.; Andresen, V.; Hell, S. W.
Language:English
Date of Publication (YYYY-MM-DD):2002-04-01
Title of Journal:Journal of Microscopy
Volume:206
Start Page:24
End Page:32
Review Status:Peer-review
Audience:Not Specified
Abstract / Description:We compare the axial sectioning capability of multifocal confocal and multifocal multiphoton microscopy in theory and in experiment, with particular emphasis on the background arising from the cross-talk between adjacent imaging channels. We demonstrate that a time-multiplexed non-linear excitation microscope exhibits significantly less background and therefore a superior axial resolution as compared to a multifocal single- photon confocal. system. The background becomes irrelevant for thin (< 15 ?m) and sparse fluorescent samples, in which case the confocal parallelized system exhibits similar or slightly better sectioning behaviour due to its shorter excitation wavelength. Theoretical and experimental axial responses of practically implemented microscopes are given
Free Keywords:axial resolution; confocal microscopy; multifocal multiphoton microscopy
External Publication Status:published
Document Type:Article
Communicated by:N. N.
Affiliations:MPI für biophysikalische Chemie/Abt. Stefan Hell / 200
Identifiers:URL:http://onlinelibrary.wiley.com/doi/10.1046/j.1365-...
The scope and number of records on eDoc is subject to the collection policies defined by each institute - see "info" button in the collection browse view.