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          Institute: MPI für biophysikalische Chemie     Collection: Neurobiologie (Prof. Reinhard Jahn)     Display Documents

ID: 17129.0, MPI für biophysikalische Chemie / Neurobiologie (Prof. Reinhard Jahn)
Differential expression of receptors for Shiga and Cholera toxin is regulated by the cell cycle
Authors:Majoul, I.; Schmidt, T.; Pomasanova, M.; Boutkevich, E.; Kozlov, Y.; Soeling, H. D.
Date of Publication (YYYY-MM-DD):2002-02-15
Title of Journal:Journal of Cell Science
Issue / Number:4
Start Page:817
End Page:826
Review Status:Peer-review
Audience:Not Specified
Abstract / Description:Cholera and Shiga toxin bind to the cell surface via glycolipid receptors GM1 and Gb3, respectively. Surprisingly, the majority of Vero cells from a non-synchronized population bind either Cholera or Shiga toxin but not both toxins. The hypothesis that the differential expression of toxin receptors is regulated by the cell cycle was tested. We find that Cholera toxin binds preferentially in G0/G1, with little binding through S-phase to telophase, whereas Shiga toxin binds maximally through G2 to telophase but does not bind during G0/G1 and S-phase. The changes result from the corresponding changes in Gb3 and GM1 synthesis, not from variations of receptor transport to the cell surface. The changes do not reflect competition of Gb3 and GM1 synthesis for lactosylceramide. Cells as diverse as Vero cells, PC12 cells and astrocytes show the same cell-cycle- dependent regulation of glycosphingolipid receptors, suggesting that this novel phenomenon is based on a conserved regulatory mechanism.
Free Keywords:AB5-toxins; glycosphingolipid receptors; cell cycle; Golgi
Comment of the Author/Creator:Date: 2002, FEB 15
External Publication Status:published
Document Type:Article
Communicated by:N. N.
Affiliations:MPI für biophysikalische Chemie/Abt. Reinhard Jahn / 190
MPI für biophysikalische Chemie/AG Hans-Dieter Söling
External Affiliations:VA Engelhardt Mol Biol Inst, Dept Mol Biol, Moscow 117984, Russia; Oslo Univ Ctr Med Studies Moscow, Moscow, Russia; Inst Med, Minsk, Byelarus
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