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          Institute: MPI für biophysikalische Chemie     Collection: Abteilungsunabhängige Arbeitsgruppen     Display Documents



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ID: 17132.0, MPI für biophysikalische Chemie / Abteilungsunabhängige Arbeitsgruppen
Highly efficient single molecule detection in different micro and submicrometer channels with cw-excitation
Authors:Dorre, K.; Stephan, J.; Eigen, M.
Language:English
Date of Publication (YYYY-MM-DD):2001
Title of Journal:Single Molecules
Volume:2
Issue / Number:3
Start Page:165
End Page:175
Review Status:Peer-review
Audience:Not Specified
Abstract / Description:In this article we describe a detailed comparison between single molecule detection in three different types of microchannels. The overall detection efficiencies and the signal-to-noise ratios are compared. A new data evaluation technique that is used is explained in detail. The average number of photons per molecule is calculated from the measurements and the detection properties of each channel with respect to the diffusional characteristics of the dye molecules are investigated. From the detection efficiencies advantages and drawbacks of the individual methods are elucidated as well as possible applications of the techniques are illustrated.
Comment of the Author/Creator:Date: 2001
External Publication Status:published
Document Type:Article
Communicated by:N. N.
Affiliations:MPI für biophysikalische Chemie/EG Manfred Eigen
External Affiliations:Ecole Polytech Fed Lausanne, PSE B, GNOTHIS SA, CH-1015; Lausanne, Switzerland
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