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          Institute: MPI für molekulare Genetik     Collection: Department of Human Molecular Genetics     Display Documents



  history
ID: 173613.0, MPI für molekulare Genetik / Department of Human Molecular Genetics
Mutations at the mouse ichthyosis locus are within the lamin B receptor gene: a single gene model for human Pelger-Huet anomaly
Authors:Shultz, Leonhard D.; Lyons, Bonnie L.; Burzenski, Lisa M.; Gott, Bruce; Samuels, Rebecca; Schweitzer, Peter A.; Dreger, Christine; Herrmann, Harald; Kalscheuer, Vera; Olins, Ada L.; Olins, Donald E.; Sperling, Karl; Hoffmann, Katrin
Language:English
Date of Publication (YYYY-MM-DD):2003-01
Title of Journal:Human Molecular Genetics
Journal Abbrev.:Hum. Mol. Genet.
Volume:12
Issue / Number:1
Start Page:61
End Page:69
Copyright:© 2003 Oxford University Press
Review Status:not specified
Audience:Experts Only
Abstract / Description:The nature of the wild-type gene product at the mouse ichthyosis (ic) locus has been of great interest because mutations at this locus cause marked abnormalities in nuclear heterochromatin, similar to those observed in Pelger–Huët anomaly (PHA). We recently found that human PHA is caused by mutations in the gene (LBR) encoding lamin B receptor, an evolutionarily conserved inner nuclear membrane protein involved in nuclear assembly and chromatin binding. Mice homozygous for deleterious alleles at the ichthyosis (ic) locus present with a blood phenotype similar to PHA, and develop other phenotypic abnormalities, including alopecia, variable expression of syndactyly and hydrocephalus. The ic locus on mouse chromosome 1 shares conserved synteny with the chromosomal location of the human LBR locus on human chromosome 1. In this study, we identified one nonsense (815ins) and two frameshift mutations (1088insCC and 1884insGGAA) within the Lbr gene of mice homozygous for either of three independent mutations (ic, icJ and ic4J, respectively) at the ichthyosis locus. These allelic mutations are predicted to result in truncated or severely impaired LBR protein. Our studies of mice homozygous for the icJ mutation revealed a complete loss of LBR protein as shown by immunofluorescence microscopy and immunoblotting. The findings provide the molecular basis for the heterochromatin clumping and other distinct phenotypes caused by ic mutations. These spontaneous Lbr mutations confirm the molecular basis of human PHA and provide a small animal model for determination of the precise function of LBR in normal and pathological states.
Comment of the Author/Creator:Date: 2003, Jan
External Publication Status:published
Document Type:Article
Version Comment:Automatic journal name synchronization
Communicated by:Hans-Hilger Ropers
Affiliations:MPI für molekulare Genetik
External Affiliations:Jackson Lab, Bar Harbor, ME 04609 USA.; German Canc Res Ctr, D-6900 Heidelberg, Germany.; Max Planck Inst Mol Genet, Berlin, Germany.; Bowdoin Coll, Brunswick, ME 04011 USA.; Humboldt Univ, Charite, Inst Human Genet, Berlin, Germany.; Humboldt Univ, Charite, Franz Volhard Clin, Berlin, Germany.; Humboldt Univ, Max Delbruck Ctr Mol Med, Gene Mapping Ctr, Berlin, Germany.
Identifiers:ISI:000180161000007 [ID No:1]
ISSN:0964-6906 [ID No:2]
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