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          Institute: MPI für molekulare Genetik     Collection: Sequencing Group     Display Documents

ID: 173936.0, MPI für molekulare Genetik / Sequencing Group
Characterization of a spontaneous nonmagnetic mutant of Magnetospirillum gryphiswaldense reveals a large deletion comprising a putative magnetosome island
Authors:Schubbe, Sabrina; Kube, Michael; Scheffel, André; Wawer, Cathrin; Heyen, Udo; Meyerdierks, Anke; Madkour, Mohamed H.; Mayer, Frank; Reinhardt, Richard; Schuler, Dirk
Date of Publication (YYYY-MM-DD):2003-10
Title of Journal:Journal of Bacteriology
Journal Abbrev.:J. Bacteriol.
Issue / Number:19
Start Page:5779
End Page:5790
Copyright:© 2003, American Society for Microbiology.
Review Status:not specified
Audience:Experts Only
Abstract / Description:Frequent spontaneous loss of the magnetic phenotype was observed in stationary-phase cultures of the magnetotactic bacterium Magnetospirillum gryphiswaldense MSR-1. A nonmagnetic mutant, designated strain MSR-1B, was isolated and characterized. The mutant lacked any structures resembling magnetosome crystals as well as internal membrane vesicles. The growth of strain MSR-1B was impaired under all growth conditions tested, and the uptake and accumulation of iron were drastically reduced under iron-replete conditions. A large chromosomal deletion of approximately 80 kb was identified in strain MSR-1B, which comprised both the entire mamAB and mamDC clusters as well as further putative operons encoding a number of magnetosome-associated proteins. A bacterial artificial chromosome clone partially covering the deleted region was isolated from the genomic library of wild-type M. gryphiswaldense. Sequence analysis of this fragment revealed that all previously identified mam genes were closely linked with genes encoding other magnetosome-associated proteins within less than 35 kb. In addition, this region was remarkably rich in insertion elements and harbored a considerable number of unknown gene families which appeared to be specific for magnetotactic bacteria. Overall, these findings suggest the existence of a putative large magnetosome island in M. gryphiswaldense and other magnetotactic bacteria.
Comment of the Author/Creator:Date: 2003, Oct
External Publication Status:published
Document Type:Article
Version Comment:Automatic journal name synchronization
Communicated by:Richard Reinhardt
Affiliations:MPI für molekulare Genetik
External Affiliations:Max Planck Inst Marine Mikrobiol, D-28359 Bremen, Germany.; Max Planck Inst Mol Genet, D-14195 Berlin, Germany.; Univ Gottingen, Inst Mikrobiol & Genet, D-37077 Gottingen, Germany.
Identifiers:ISI:000185493500014 [ID No:1]
ISSN:0021-9193 [ID No:2]
DOI:10.1128/JB.185.19.5779-5790.2003 [ID No:3]
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