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          Institute: MPI für molekulare Genetik     Collection: Department of Computational Molecular Biology     Display Documents

ID: 175667.0, MPI für molekulare Genetik / Department of Computational Molecular Biology
Analysis of CREM-dependent gene expression during
mouse spermatogenesis
Authors:Beißbarth, Tim; Borisevich, Igor; Hörlein, Andreas; Kenzelmann, Marc; Hergenhahn, Manfred; Klewe-Nebenius, Annette; Klären, Ralf; Korn, Bernhard; Schmid, Wolfgang; Vingron, Martin; Schütz, Günther
Date of Publication (YYYY-MM-DD):2003-09-17
Title of Journal:Molecular and Cellular Endocrinology
Journal Abbrev.:Mol Cell Endocrinol
Issue / Number:1-2
Start Page:29
End Page:39
Copyright:© 2003 Elsevier Ireland Ltd
Review Status:not specified
Audience:Experts Only
Abstract / Description:The transcription factors CREM, CREB, and ATF-1 constitute a subfamily of -Zip transcription factors. Several different kinase cascades regulate the activity of these proteins. The activator splice–isoform CREM is specifically and highly expressed in post-meiotic germ cells during mouse spermatogenesis. Male mice lacking CREM expression are sterile because of stage-specific arrest of sperm maturation as the spermatids undergo apoptosis.
In order to characterize the genes that are controlled by CREM during post-meiotic differentiation of round spermatids, we compared the expression levels of mRNA prepared from testes of wild-type and CREM-deficient mice by suppression subtractive hybridization (SSH) and affymetrix oligonucleotide arrays.
A set of 956 unique sequences found in the CREM SSH library was further characterized by generating stage-specific expression profiles during spermatogenesis by hybridization with cDNAfrom pre-pubertal mice at defined stages of spermatogenesis using nylonDNAarrays. The resulting expression profiles were arranged in a linear order according to similarity in their profile shapes to find co-regulation of functionally related genes.
Our data shows that a large number of genes are transcriptionally activated in round spermatids when CREM activity is maximal, including functional groups like transcription factors, proteins involved in signal transduction, and metabolic enzymes, therefore providing novel information of post-meiotic expression of many known as well as novel genes that are either directly or indirectly influenced by CREM expression.
Free Keywords:Mouse testis; CREM; Gene-expression; SSH; Microarray
External Publication Status:published
Document Type:Article
Version Comment:Automatic journal name synchronization
Communicated by:Martin Vingron
Affiliations:MPI für molekulare Genetik
External Affiliations:Molecular Biology of the Cell 1, German Cancer Research Center, DKFZ, Im Neuenheimer Feld 280, D-69120 Heidelberg, Germany
Theoretical Bioinformatics, DKFZ, Im Neuenheimer Feld 280, D-69120 Heidelberg, Germany
Genetic Alterations in Carcinogenesis, DKFZ, Im Neuenheimer Feld 280, D-69120 Heidelberg, Germany
RZPD—Ressourcenzentrum für die Genomforschung, INF 506, D-69120 Heidelberg, Germany
Identifiers:ISSN:0303-7207 [ID No:1]
DOI:10.1016/j.mce.2003.09.023 [ID No:2]
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