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          Institute: MPI für Entwicklungsbiologie     Collection: Abteilungsunabhängige Arbeitsgruppen     Display Documents



ID: 176119.0, MPI für Entwicklungsbiologie / Abteilungsunabhängige Arbeitsgruppen
Reconstruction of the central carbohydrate metabolism of Thermoproteus tenax by use of genomic and biochemical data
Authors:Siebers, B.; Tjaden, B.; Michalke, K.; Dorr, C.; Ahmed, H.; Zaparty, M.; Gordon, P.; Sensen, C. W.; Zibat, A.; Klenk, H. P.; Schuster, Stephan; Hensel, R.
Language:English
Date of Publication (YYYY-MM-DD):2004-04
Title of Journal:J Bacteriol
Volume:186
Issue / Number:7
Start Page:2179
End Page:2194
Sequence Number of Article:15028704
Review Status:not specified
Audience:Not Specified
Abstract / Description:The hyperthermophilic, facultatively heterotrophic crenarchaeum Thermoproteus tenax was analyzed using a low-coverage shotgun-sequencing approach. A total of 1.81 Mbp (representing 98.5% of the total genome), with an average gap size of 100 bp and 5.3-fold coverage, are reported, giving insights into the genome of T. tenax. Genome analysis and biochemical studies enabled us to reconstruct its central carbohydrate metabolism. T. tenax uses a variant of the reversible Embden-Meyerhof-Parnas (EMP) pathway and two different variants of the Entner-Doudoroff (ED) pathway (a nonphosphorylative variant and a semiphosphorylative variant) for carbohydrate catabolism. For the EMP pathway some new, unexpected enzymes were identified. The semiphosphorylative ED pathway, hitherto supposed to be active only in halophiles, is found in T. tenax. No evidence for a functional pentose phosphate pathway, which is essential for the generation of pentoses and NADPH for anabolic purposes in bacteria and eucarya, is found in T. tenax. Most genes involved in the reversible citric acid cycle were identified, suggesting the presence of a functional oxidative cycle under heterotrophic growth conditions and a reductive cycle for CO2 fixation under autotrophic growth conditions. Almost all genes necessary for glycogen and trehalose metabolism were identified in the T. tenax genome.
External Publication Status:published
Document Type:Article
Affiliations:MPI für Entwicklungsbiologie/Abteilungsunabhängige Arbeitsgruppen
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