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          Institute: MPI für Entwicklungsbiologie     Collection: Abteilungsunabhängige Arbeitsgruppen     Display Documents



ID: 176143.0, MPI für Entwicklungsbiologie / Abteilungsunabhängige Arbeitsgruppen
The Brain-specific Double-stranded RNA-binding Protein Staufen2: NUCLEOLAR ACCUMULATION AND ISOFORM-SPECIFIC EXPORTIN-5-DEPENDENT EXPORT.
Authors:Macchi, Paolo; Brownawell, A. M.; Grunewald, Barbara; DesGroseillers, L.; Macara, I. G.; Kiebler, Michael
Date of Publication (YYYY-MM-DD):2004-07
Title of Journal:Journal of Biological Chemistry
Journal Abbrev.:J Biol Chem
Volume:279
Issue / Number:30
Start Page:31440
End Page:31444
Sequence Number of Article:15166236
Review Status:not specified
Audience:Not Specified
Abstract / Description:The mammalian double-stranded RNA-binding proteins Staufen (Stau1 and Stau2) are involved in RNA localization in polarized neurons. In contrast to the more ubiquitously expressed Stau1, Stau2 is mainly expressed in the nervous system. In Drosophila, the third double-stranded RNA-binding domain (RBD3) of Staufen is essential for RNA interaction. When conserved amino acids within the RBD3 of Stau2 were mutated to render Stau2 defective for RNA binding, the mutant Stau2 proteins accumulate predominantly in the nucleolus. This is in contrast to wild type Stau2 that mostly localizes in the cytosol. The nuclear import is dependent on a nuclear localization signal in close proximity to the RBD3. The nuclear export of Stau2 is not dependent on CRM1 but rather on Exportin-5. We show that Exportin-5 interacts with the RBD3 of wild type Stau2 in an RNA-dependent manner in vitro but not with mutant Stau2. When Exportin-5 is down-regulated by RNA interference, only the largest isoform of Stau2 (Stau2(62)) preferentially accumulates in the nucleolus. It is tempting to speculate that Stau2(62) binds RNA in the nucleus and assembles into ribonucleoparticles, which are then exported via the Exportin-5 pathway to their final destination.
External Publication Status:published
Document Type:Article
Affiliations:MPI für Entwicklungsbiologie/Abteilungsunabhängige Arbeitsgruppen
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