MPI für molekulare Genetik / Research Group Development and Disease |
|A single amphioxus and sea urchin runt-gene suggests that runt-gene duplications occurred in early chordate evolution|
|Authors:||Stricker, S.; Poustka, A. J.; Wiecha, U.; Stiege, A.; Hecht, J.; Panopoulou, G.; Vilcinskas, A.; Mundlos, S.; Seitz, V.|
|Date of Publication (YYYY-MM-DD):||2003-09|
|Title of Journal:||Developmental and Comparative Immunology|
|Journal Abbrev.:||Dev. Comp. Immunol.|
|Issue / Number:||8|
|Copyright:||© 2003 Elsevier Science Ltd|
|Review Status:||not specified|
|Abstract / Description:||Runt-homologous molecules are characterized by their DNA binding runt-domain which is highly conserved within bilaterians. The three mammalian runt-genes are master regulators in cartilage/bone formation and hematopoiesis. Historically these features evolved in Craniota and might have been promoted by runt-gene duplication events. The purpose of this study was therefore to investigate how many runt-genes exist in the stem species of chordates, by analyzing the number of runt-genes in what is likely to be the closest living relative of Craniota—amphioxus. To acquire further insight into the possible role of runt-genes in early chordate evolution we have determined the number of runt-genes in sea urchins and have analyzed the runt-expression pattern in this species.
Our findings demonstrate the presence of a single runt-gene in amphioxus and sea urchin, which makes it highly likely that the stem species of chordates harbored only a single runt-gene. This suggests that runt-gene duplications occurred later in chordate phylogeny, and are possibly also associated with the evolution of features such as hematopoiesis, cartilage and bone development.
In sea urchin embryos runt-expression involves cells of endodermal, mesodermal and ectodermal origin. This complex pattern of expression might reflect the multiple roles played by runt-genes in mammals. A strong runt-signal in the gastrointestinal tract of the sea urchin is in line with runt-expression in the intestine of nematodes and in the murine gastrointestinal tract, and seems to be one of the phylogenetically ancient runt-expression domains.
|Free Keywords:||runt; amphioxus; sea urchin; cartilage; bone; hematopoiesis; gene duplication; chordates|
|Comment of the Author/Creator:||Date: 2003, Sep|
|External Publication Status:||published|
|Version Comment:||Automatic journal name synchronization|
|Communicated by:||Stefan Mundlos|
|Affiliations:||MPI für molekulare Genetik|
|External Affiliations:||Max Planck Inst Mol Genet, D-14195 Berlin, Germany
Univ Potsdam, Syst Zool & Evolutionary Biol, D-14417 Potsdam, Germany
|Identifiers:||ISI:000183906600003 [ID No:1] |
ISSN:0145-305X [ID No:2]
DOI:10.1016/S0145-305X(03)00037-5 [ID No:3]