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          Institute: MPI für molekulare Genetik     Collection: Research Group Development and Disease     Display Documents

ID: 194817.0, MPI für molekulare Genetik / Research Group Development and Disease
Identification of immunorelevant genes from greater wax moth (Galleria mellonella) by a subtractive hybridization approach
Authors:Seitz, Volkhard; Clermont, A.; Wedde, M.; Hummel, M.; Vilcinskas, A.; Schlatterer, K.; Podsiadlowski, L.
Date of Publication (YYYY-MM-DD):2003-03
Title of Journal:Developmental and Comparative Immunology
Journal Abbrev.:Dev. Comp. Immunol.
Issue / Number:3
Start Page:207
End Page:215
Copyright:© 2002 Elsevier Science Ltd.
Review Status:not specified
Audience:Experts Only
Abstract / Description:In this study we have analyzed bacterial lipopolysaccharide (LPS) induced genes in hemocytes of the Lepidopteran species Galleria mellonella using subtractive hybridization, followed by suppressive PCR. We have found genes that show homologies to molecules, such as gloverin, peptidoglycan recognition proteins and transferrin known to be involved in immunomodulation after bacterial infection in other species. In addition, a few molecules previously not described in the innate immune reactions were detected, such as a RNA binding molecule and tyrosine hydroxylase. Furthermore, the full-length cDNA of a LPS-induced molecule with six toxin-2-like domains is described to be a promising candidate to further elucidate the relationship between toxin- and defensin-like domains in arthropod host defense.
Free Keywords:Galleria mellonella, lipopolysaccharide, subtractive hybridization, real-time PCR, innate immunity, gloverin, peptidoglycan recognition protein, toxin-2
Comment of the Author/Creator:Date: 2003, MAR
External Publication Status:published
Document Type:Article
Version Comment:Automatic journal name synchronization
Communicated by:Stefan Mundlos
Affiliations:MPI für molekulare Genetik
External Affiliations:Max Planck Inst Mol Genet, D-14195 Berlin, Germany.; Free Univ Berlin, Inst Pathol, D-12200 Berlin, Germany.; Univ Potsdam, D-14417 Potsdam, Germany.; Free Univ Berlin, Inst Clin Chem & Pathobiochem, D-12200 Berlin, Germany.; Free Univ Berlin, Inst Zool, D-14195 Berlin, Germany.
Identifiers:ISI:000181301800005 [ID No:1]
ISSN:0145-305X [ID No:2]
DOI:10.1016/S0145-305X(02)00097-6 [ID No:3]
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