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          Institute: MPI für Herz- und Lungenforschung (W. G. Kerckhoff Institut)     Collection: Publikationen des W. G. Kerckhoff-Instituts     Display Documents

ID: 199038.0, MPI für Herz- und Lungenforschung (W. G. Kerckhoff Institut) / Publikationen des W. G. Kerckhoff-Instituts
Identification of differentially expressed genes like cofilin2 in growing collateral arteries
Authors:Boengler, K.; Pipp, F.; Broich, K.; Fernandez, B.; Schaper, W.; Deindl, E.
Date of Publication (YYYY-MM-DD):2003-01-17
Title of Journal:Biochem Biophys Res Commun
Issue / Number:3
Start Page:751
End Page:756
Review Status:not specified
Audience:Not Specified
Abstract / Description:Arteriogenesis, the growth of pre-existing collateral arteries, can be induced in rabbits by occlusion of the femoral artery. In order to analyze the differential gene expression in arteriogenesis, cDNA of collateral arteries 24h after femoral occlusion or sham operation was subjected to suppression subtractive hybridization (SSH). We demonstrated an upregulation of the U6 snRNA binding protein Lsm5, cytochrome b, an expressed sequence tag, and the actin-depolymerizing factor cofilin2 mRNA in collateral arteries 24h after femoral ligation. For cofilin2, we also detected an increase in the protein level and a localization predominantly in smooth muscle cells of collaterals. Simultaneously with the upregulation of cofilin2 we found a downregulation of the alpha-smooth muscle actin mRNA in growing collateral arteries. In summary, our data showed an augmented expression level of genes contributing to different fundamental processes of arteriogenesis.
Free Keywords:Actins/biosynthesis/genetics
; Animals
; Arteries/*growth & development/*metabolism
; Blotting, Northern
; Collateral Circulation/genetics/*physiology
; *Gene Expression Profiling
; Hindlimb/blood supply
; Ligation
; Microfilament Proteins/*biosynthesis/genetics
; Models, Animal
; Muscle, Skeletal/blood supply/metabolism
; Neovascularization, Physiologic/genetics
; RNA, Messenger/metabolism
; Rabbits
; Up-Regulation
External Publication Status:published
Document Type:Article
Communicated by:N. N.
Affiliations:MPI für physiologische und klinische Forschung
Identifiers:URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=... [ID No:1]
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