Please note that eDoc will be permanently shut down in the first quarter of 2021!      Home News About Us Contact Contributors Disclaimer Privacy Policy Help FAQ

Home
Search
Quick Search
Advanced
Fulltext
Browse
Collections
Persons
My eDoc
Session History
Login
Name:
Password:
Documentation
Help
Support Wiki
Direct access to
document ID:


          Institute: MPI für Herz- und Lungenforschung (W. G. Kerckhoff Institut)     Collection: Publikationen des W. G. Kerckhoff-Instituts     Display Documents



  history
ID: 199084.0, MPI für Herz- und Lungenforschung (W. G. Kerckhoff Institut) / Publikationen des W. G. Kerckhoff-Instituts
Inhibition of collateral artery growth by mibefradil: possible role of volume-regulated chloride channels
Authors:Ziegelhoeffer, T.; Scholz, D.; Friedrich, C.; Helisch, A.; Wagner, S.; Fernandez, B.; Schaper, W.
Date of Publication (YYYY-MM-DD):2003
Title of Journal:Endothelium
Volume:10
Issue / Number:4-5
Start Page:237
End Page:246
Review Status:not specified
Audience:Not Specified
Abstract / Description:Endothelial cell swelling is one of the earliest hallmarks of arteriogenesis, the growth and maturation of collaterals. Mibefradil was found to block endothelial Cl(-) channels that control the volume of endothelial cells. Thus the authors investigated whether the blockade of volume-controlling endothelial cell channels would translate into an inhibition of arteriogenesis. In BALB/c mice, the right femoral artery was ligated and the animals received either mibefradil or solvent (phosphate-buffered saline [PBS]) via osmotic minipumps. Laser Doppler perfusion ratio (R/L) of ligated versus nonligated distal hindlimb increased from 0.06 ± 0.01 (immediately after ligation) to 0.25 ± 0.02 (day 7) in the PBS group and only from 0.07 ± 0.02 to 0.13 ± 0.02 in the mibefradil group (p <.01). Collateral artery diameters were significantly smaller in the mibefradil group (61 ± 4.7 microm) versus controls (77.3 ± 0.9 microm) (p <.05). Relative hemoglobin oxygen saturation measurements confirmed these findings (p <.02). The inhibition of arteriogenesis in the mibefradil group suggests that endothelial Cl(-) channels are involved in the initiation of arteriogenesis.
Free Keywords:Animals
; Arteries/drug effects/*growth & development/ultrastructure
; Chloride Channels/*antagonists & inhibitors/physiology
; Collateral Circulation/*drug effects
; Coronary Angiography
; Endothelium, Vascular/cytology
; Erythrocyte Indices
; Femoral Artery/growth & development
; Mibefradil/*pharmacology
; Mice
; Mice, Inbred BALB C
; Oxygen Consumption
; Support, Non-U.S. Gov't
External Publication Status:published
Document Type:Article
Communicated by:N. N.
Affiliations:MPI für physiologische und klinische Forschung
Identifiers:URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=... [ID No:1]
The scope and number of records on eDoc is subject to the collection policies defined by each institute - see "info" button in the collection browse view.