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          Institute: MPI für Herz- und Lungenforschung (W. G. Kerckhoff Institut)     Collection: Publikationen des W. G. Kerckhoff-Instituts     Display Documents

ID: 199084.0, MPI für Herz- und Lungenforschung (W. G. Kerckhoff Institut) / Publikationen des W. G. Kerckhoff-Instituts
Inhibition of collateral artery growth by mibefradil: possible role of volume-regulated chloride channels
Authors:Ziegelhoeffer, T.; Scholz, D.; Friedrich, C.; Helisch, A.; Wagner, S.; Fernandez, B.; Schaper, W.
Date of Publication (YYYY-MM-DD):2003
Title of Journal:Endothelium
Issue / Number:4-5
Start Page:237
End Page:246
Review Status:not specified
Audience:Not Specified
Abstract / Description:Endothelial cell swelling is one of the earliest hallmarks of arteriogenesis, the growth and maturation of collaterals. Mibefradil was found to block endothelial Cl(-) channels that control the volume of endothelial cells. Thus the authors investigated whether the blockade of volume-controlling endothelial cell channels would translate into an inhibition of arteriogenesis. In BALB/c mice, the right femoral artery was ligated and the animals received either mibefradil or solvent (phosphate-buffered saline [PBS]) via osmotic minipumps. Laser Doppler perfusion ratio (R/L) of ligated versus nonligated distal hindlimb increased from 0.06 ± 0.01 (immediately after ligation) to 0.25 ± 0.02 (day 7) in the PBS group and only from 0.07 ± 0.02 to 0.13 ± 0.02 in the mibefradil group (p <.01). Collateral artery diameters were significantly smaller in the mibefradil group (61 ± 4.7 microm) versus controls (77.3 ± 0.9 microm) (p <.05). Relative hemoglobin oxygen saturation measurements confirmed these findings (p <.02). The inhibition of arteriogenesis in the mibefradil group suggests that endothelial Cl(-) channels are involved in the initiation of arteriogenesis.
Free Keywords:Animals
; Arteries/drug effects/*growth & development/ultrastructure
; Chloride Channels/*antagonists & inhibitors/physiology
; Collateral Circulation/*drug effects
; Coronary Angiography
; Endothelium, Vascular/cytology
; Erythrocyte Indices
; Femoral Artery/growth & development
; Mibefradil/*pharmacology
; Mice
; Mice, Inbred BALB C
; Oxygen Consumption
; Support, Non-U.S. Gov't
External Publication Status:published
Document Type:Article
Communicated by:N. N.
Affiliations:MPI für physiologische und klinische Forschung
Identifiers:URL: [ID No:1]
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