Please note that eDoc will be permanently shut down in the first quarter of 2021!      Home News About Us Contact Contributors Disclaimer Privacy Policy Help FAQ

Quick Search
My eDoc
Session History
Support Wiki
Direct access to
document ID:

          Institute: MPI für Kohlenforschung     Collection: Publikationen MPI für Kohlenforschung     Display Documents

ID: 20470.0, MPI für Kohlenforschung / Publikationen MPI für Kohlenforschung
Immobilization of chiral enzyme inhibitors on solid supports by amide-forming coupling and olefin metathesis
Authors:Reetz, M. T.; Rüggeberg, C. J.; Dröge, M. J.; Quax, W. J.
Date of Publication (YYYY-MM-DD):2002-10-14
Title of Journal:Tetrahedron
Journal Abbrev.:Tetrahedron
Issue / Number:42
Start Page:8465
End Page:8473
Review Status:Peer-review
Audience:Experts Only
Abstract / Description:The question whether phage display can be used as a selection method in the directed evolution of enantioselective enzymes has not been answered satisfactorily to date. In order to be able to test this in a specific case, namely in the hydrolytic kinetic resolution of the acetate derived from alpha,beta- isopropylideneglycerol (IPG) catalyzed by the lipase from Bacillus subtilis, suicide enzyme inhibitors anchored on porous glass or polymer beads were designed and synthesized. These are immobilized phosphonates, which bear a leaving group and also contain the chiral substrate (D) and (L)-IPG, Modified SIRAN((R)) (porous glass) and Tentagel((R)) (polymer) were chosen as carriers, attachment occurring via amide-forming coupling or Ru-catalyzed olefin metathesis. Initial lipase inhibition studies are also reported. (C) 2002 Elsevier Science Ltd. All rights reserved.
Free Keywords:biotransformations; olefin metathesis; asymmetric catalysis; lipase; phage display
Comment of the Author/Creator:Date: 2002, OCT 14
External Publication Status:published
Document Type:Article
Communicated by:N. N.
Affiliations:MPI für Kohlenforschung
External Affiliations:Univ Groningen, Ctr Pharm, Dept Pharmaceut Biol, NL-9713 AV Groningen, Netherlands
Identifiers:ISI:000178624700010 [ID No:1]
ISSN:0040-4020 [ID No:2]
The scope and number of records on eDoc is subject to the collection policies defined by each institute - see "info" button in the collection browse view.