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          Institute: MPI für biophysikalische Chemie     Collection: Abteilungsunabhängige Arbeitsgruppen     Display Documents

ID: 20766.0, MPI für biophysikalische Chemie / Abteilungsunabhängige Arbeitsgruppen
Sr2+ binding to the Ca2+ binding site of the synaptotagmin 1 C2B domain triggers fast exocytosis without stimulating SNARE interactions
Authors:Shin, O. H.; Rhee, J. S.; Tang, J.; Sugita, S.; Rosenmund, C.; Suedhof, T. C.
Date of Publication (YYYY-MM-DD):2003-01-09
Title of Journal:Neuron
Issue / Number:1
Start Page:99
End Page:108
Review Status:Peer-review
Audience:Not Specified
Abstract / Description:Sr2+ triggers neurotransmitter release similar to Ca2+, but less efficiently. We now show that in synaptotagmin 1 knockout mice, the fast component of both Ca2+- and Sr2+-induced release is selectively impaired, suggesting that both cations partly act by binding to synaptotagmin 1. Both the C(2)A and the C2B domain of synaptotagmin 1 bind Ca2+ in phospholipid complexes, but only the C2B domain forms Sr2+/phospholipid complexes; therefore, Sr2+ binding to the C2B domain is sufficient to trigger fast release, although with decreased efficacy. Ca2+ induces binding of the synaptotagmin C, domains to SNARE proteins, whereas Sr2+ even at high concentrations does not. Thus, triggering of the fast component of release by Sr2+ as a Ca2+ agonist involves the formation of synaptotagmin/ phospholipid complexes, but does not require stimulated SNARE binding.
Comment of the Author/Creator:Date: 2003, JAN 9
External Publication Status:published
Document Type:Article
Communicated by:N. N.
Affiliations:MPI für biophysikalische Chemie/AG Christian Rosenmund
External Affiliations:Univ Texas, SW Med Ctr, Ctr Basic Neurosci, Dept Mol Genet,; Dallas, TX 75390 USA; Univ Texas, SW Med Ctr, Ctr Basic Neurosci, Dept Mol Genet, Dallas, TX 75390 USA; Univ Texas, SW Med Ctr, Howard Hughes Med Inst, Dallas, TX 75390 USA
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