Home News About Us Contact Contributors Disclaimer Privacy Policy Help FAQ

Home
Search
Quick Search
Advanced
Fulltext
Browse
Collections
Persons
My eDoc
Session History
Login
Name:
Password:
Documentation
Help
Support Wiki
Direct access to
document ID:


          Institute: MPI für Dynamik komplexer technischer Systeme     Collection: Bioprocess Engineering     Display Documents



  history
ID: 207697.0, MPI für Dynamik komplexer technischer Systeme / Bioprocess Engineering
Glutamine-free media : use of pyruvate to avoid accumulation of ammonia in mammalian cell culture
Authors:Genzel, Y.; Alt, R.; Reichl, U.
Language:English
Name of Conference/Meeting:ESBES-5 : European Symposium on Biochemical Engineering Science
Place of Conference/Meeting:Stuttgart, Germany
(Start) Date of Event 
 (YYYY-MM-DD):
2004-09-08
End Date of Conference/Meeting 
 (YYYY-MM-DD):
2004-09-11
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:Glutamine is known to be one of the mayor energy sources in mammalian cell culture technology together with glucose. Different pathways for glutamine metabolism are possible, resulting in different energy and ammonia output. Ammonia can limit cell growth and product formation. Ideas to reduce ammonia formation are numerous. Here we present new aspects on the role of glutamine in mammalian cell culture media. We will show that the replacement of glutamine by pyruvate is supporting growth of different commercial cell lines (MDCK, BHK21, CHO) in serum containing and serum free media without ammonia production. No further additives, like peptides or hydrolysates are required. In addition, less lactate, another cell growth inhibitor is produced as less glucose is used. The use of this new medium formulation is presented in detail, considering the metabolism of MDCK cells in an influenza vaccine production process in roller bottles and large-scale microcarrier cultures as an example. The metabolite profiles for glucose, lactate, glutamine, ammonium, glutamate, pyruvate and extracellular amino acids will be analyzed. Metabolic pathways, which allow the cells to grow without glutamine, will be discussed. Further insights on the glutaminolysis are given by comparing metabolite profiles from variations of this new medium formulation. Additionally the impact on virus yield will be reported.
Document Type:Poster
Communicated by:Udo Reichl
Affiliations:MPI für Dynamik komplexer technischer Systeme/Bioprocess Engineering
Identifiers:LOCALID:1016
The scope and number of records on eDoc is subject to the collection policies defined by each institute - see "info" button in the collection browse view.