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          Institute: MPI für Dynamik komplexer technischer Systeme     Collection: Bioprocess Engineering     Display Documents



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ID: 207990.0, MPI für Dynamik komplexer technischer Systeme / Bioprocess Engineering
Metabolism of MDCK Cells during cell growth and influenza virus production in large-scale microcarrier culture
Authors:Genzel, Y.; Behrendt, I.; König, S.; Sann, H.; Reichl, U.
Language:English
Date of Publication (YYYY-MM-DD):2004
Title of Journal:Vaccine
Volume:22
Start Page:2202
End Page:2208
Review Status:not specified
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:The production of equine influenza in Madin Darby canine kidney (MDCK) cells in large-scale microcarrier culture is described with detailed on- and off-line analytical data during cell growth and virus replication. Metabolite concentration profiles for glucose, glutamine, lactate and ammonium are shown. Lactate and ammonium concentrations were always below inhibiting levels. Concentration profiles for essential and non-essential amino acids of the cell culture medium are discussed. During cell growth proline was released into the medium with a significant rate while two amino acids, serine and methionine were almost depleted. After infection, virus titer increased after a delay of 10 16 h whereas first changes in amino acid metabolism could be observed within 4 h post-infection. Here, glutamate and aspartate increase correlated to virus release kinetics, indicating cell disruption and apoptosis. Starting with a moi of 0.025 resulted in a maximum virus yield of 2.4log HA/100 µ l at 44 h post-infection.
External Publication Status:published
Document Type:Article
Communicated by:Udo Reichl
Affiliations:MPI für Dynamik komplexer technischer Systeme/Bioprocess Engineering
Identifiers:LOCALID:921 11/04
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