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          Institute: MPI für medizinische Forschung     Collection: Abteilung Zellphysiologie     Display Documents



ID: 22152.0, MPI für medizinische Forschung / Abteilung Zellphysiologie
Location of a δ-subunit region determining ion transport through the acetylcholine receptor channel
Translation of Title:Location of a δ-subunit region determining ion transport through the acetylcholine receptor channel
Authors:Imoto, K.; Methfessel, Christoph; Sakmann, Bert; Mishina, M.; Mori, Y.; Konno, T.; Fukuda, K.; Kurasaki, M.; Bujo, Hideaki; Fujita, Y.; Numa, S.
Language:English
Date of Publication (YYYY-MM-DD):1986
Title of Journal:Nature
Journal Abbrev.:Nat.
Volume:324
Issue / Number:6098
Start Page:670
End Page:674
Review Status:Peer-review
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:The combination of complementary DNA expression and single-channel current analysis provides a powerful tool for studying the structure-function relationship of the nicotinic acetylcholine receptor (AChR) (refs 1-5). We have previously shown that AChR channels consisting of subunits from different species, expressed in the surface membrane of Xenopus oocytes, can be used to relate functional properties to individual subunits. Here we report that, in extracellular solution of low divalent cation concentration, the bovine AChR channel has a smaller conductance than the Torpedo AChR channel. Replacement of the delta-subunit of the Torpedo AChR by the bovine delta-subunit makes the channel conductance similar to that of the bovine AChR channel. To locate the region in the delta-subunit responsible for this difference, we have constructed chimaeric delta-subunit cDNAs with different combinations of the Torpedo and bovine counterparts. The conductances of AChR channels containing chimaeric delta-subunits suggest that a region comprising the putative transmembrane segment M2 and the adjacent bend portion between segments M2 and M3 is involved in determining the rate of ion transport through the open channel.
External Publication Status:published
Document Type:Article
Communicated by:Wulf Kaiser
Affiliations:MPI für medizinische Forschung/Abteilung Zellphysiologie/
Identifiers:URI:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=... [Abstract]
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