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          Institute: MPI für medizinische Forschung     Collection: Abteilung Molekulare Neurobiologie     Display Documents



ID: 22376.0, MPI für medizinische Forschung / Abteilung Molekulare Neurobiologie
RNA editing of AMPA receptor subunit GluR-B: a base-paired intron exon structure determine position and efficiency
Translation of Title:RNA editing of AMPA receptor subunit GluR-B: a base-paired intron exon structure determine position and efficiency
Authors:Higuchi, Miyoko; Single, Frank N.; Köhler, Martin; Sommer, B.; Sprengel, Rolf; Seeburg, Peter H.
Language:English
Date of Publication (YYYY-MM-DD):1993-12-31
Title of Journal:Cell
Journal Abbrev.:Cell
Volume:75
Issue / Number:7
Start Page:1361
End Page:1370
Review Status:Peer-review
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:A functionally critical position (Q/R site) of the AMPA receptor subunit GluR-B is controlled by RNA editing that operates in the nucleus, since in brain and clonal cell lines of neural origin, unspliced GluR-B transcripts occur edited in the Q/R site CAG codon and, additionally, in intronic adenosines. Transfection of GluR-B gene constructs into PC12 cells revealed that the proximal part of the intron downstream of the unedited exonic site is required for Q/R site editing. This intron portion contains an imperfect inverted repeat preceding a 10 nt sequence with exact complementarity to the exon centered on the unedited codon. Single nucleotide substitutions in this short intronic sequence or its exonic complement curtailed Q/R site editing, which was recovered by restoring complementarity in the respective partner strand. Base conversion in the channel-coding region of GluR-B directed by base paired sequences may be executed by a ubiquitous nuclear adenosine deaminase specific for double-stranded RNA.
External Publication Status:published
Document Type:Article
Communicated by:Wulf Kaiser
Affiliations:MPI für medizinische Forschung/Abteilung Molekulare Neurobiologie/
Identifiers:URI:http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?c... [Abstract]
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