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          Institute: MPI für medizinische Forschung     Collection: Abteilung Zellphysiologie     Display Documents



ID: 22443.0, MPI für medizinische Forschung / Abteilung Zellphysiologie
A structural determinant of differential sensitivity of cloned inward rectifier K+ channels to intracellular spermine
Translation of Title:A structural determinant of differential sensitivity of cloned inward rectifier K<SUP>+</SUP> channels to intracellular spermine
Authors:Fakler, Bernd; Brändle, Uwe; Bond, Chris T.; Glowatzki, E.; König, C.; Adelman, John P.; Zenner, H. P.; Ruppersberg, J. Peter
Language:English
Date of Publication (YYYY-MM-DD):1994-12-19
Title of Journal:FEBS Letters
Journal Abbrev.:FEBS Lett.
Volume:356
Issue / Number:2
Start Page:199
End Page:203
Review Status:Peer-review
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:Large subtype-specific differences in the sensitivity of cloned inward-rectifier K+ channels of the IRK1, BIR10 and ROMK1 subtype to being blocked by intracellular spermine (SPM) are described. It is shown, by site-directed mutagenesis, that the four orders of magnitude larger SPM sensitivity of BIR10 channels compared to ROMK1 channels may be explained by a difference in a single amino acid in the putative transmembrane segment TMII. This residue, a negatively charged glutamate in BIR10, is homologous to the residue in IRK1 and ROMK1 which has previously been shown to change gating properties and Mg2+ sensitivity. Differential block by physiological SPM concentrations is suggested as a major functional difference between subtypes of inward-rectifier K+ channels.
Free Keywords:Inward rectifier; K+ channel; Clone; Spermine; Site-directed mutagenesis
External Publication Status:published
Document Type:Article
Communicated by:Wulf Kaiser
Affiliations:MPI für medizinische Forschung/Abteilung Zellphysiologie/
Identifiers:URI:http://www.sciencedirect.com/science?_ob=ArticleUR... [Abstract]
URI:http://www.sciencedirect.com/science?_ob=MImg&_ima... [Fulltext PDF]
DOI:10.1016/0014-5793(94)01258-X
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