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          Institute: MPI für molekulare Pflanzenphysiologie     Collection: Publikationen Pflanzenphysiologie     Display Documents

ID: 224533.0, MPI für molekulare Pflanzenphysiologie / Publikationen Pflanzenphysiologie
Cell-specific protein profiling in Arabidopsis thaliana trichomes: identification of trichome-located proteins involved in sulfur metabolism and detoxification
Authors:Wienkoop, S.; Zoeller, D.; Ebert, B.; Simon-Rosin, U.; Fisahn,, J.; Glinski, M.; Weckwerth, W.
Date of Publication (YYYY-MM-DD):2004-06
Title of Journal:Phytochemistry
Journal Abbrev.:Phytochemistry
Issue / Number:11
Start Page:1641
End Page:1649
Review Status:not specified
Audience:Not Specified
Abstract / Description:Metabolite, protein, and transcript analysis at the cellular level gives unparalleled insight into the complex roles tissues play in the plant system. However, while capillary electrophoresis and PCR amplification strategies make the profiling of metabolites and transcripts in specific cell types possible, the profiling of proteins in small samples represents a bottleneck. Here for the first time protein profiling has been achieved in a specific plant cell type: The application of specific cell sampling and shotgun peptide sequencing (nano LC/MS/MS) resulted in the identification of 63 unique proteins from pooled Arabidopsis trichome cells. A complete S-adenosylmethionine pathway cluster, two S-adenosylmethionine synthase isoforms, a glutathione S-conjugate translocator and other proteins involved in sulfur metabolism and detoxification are shown to be present in these cells, in agreement with previous work done at the level of trichome transcript analysis. The technology described here brings the simultaneous identification and localization of physiologically relevant cellular proteins within reach. (C) 2004 Elsevier Ltd. All rights reserved.
Free Keywords:plant shotgun proteomics
; nano-lc/ms
; sulfur metabolism
; single cells
; mass-spectrometry
; gene-expression
; glandular trichomes
; saccharomyces-cerevisiae
; shotgun proteomics
; yeast proteome
; barley leaves
; messenger-rna
; plant-cells
; technology
External Publication Status:published
Document Type:Article
Communicated by:N. N.
Affiliations:MPI für molekulare Pflanzenphysiologie/Metabolische Netzwerke/AG Weckwerth
MPI für molekulare Pflanzenphysiologie/Zentrale Infrastrukturgruppen/AG Fisahn
External Affiliations:Max Planck Inst Mol Plant Physiol, Metab Network, D-14424 Potsdam, Germany
Max Planck Inst Mol Plant Physiol, Metab Network, D-14424 Potsdam, Germany
Identifiers:ISI:000223440400016 [ID No:1]
ISI:000223440400016 [ID No:2]
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