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          Institute: MPI für medizinische Forschung     Collection: Abteilung Zellphysiologie     Display Documents



ID: 22473.0, MPI für medizinische Forschung / Abteilung Zellphysiologie
A look at membrane patches with a scanning force microscope
Translation of Title:A look at membrane patches with a scanning force microscope
Authors:Hörber, J. K. H.; Mosbacher, Johannes; Häberle, W.; Ruppersberg, J. Peter; Sakmann, Bert
Language:English
Date of Publication (YYYY-MM-DD):1995-05
Title of Journal:Biophysical Journal
Journal Abbrev.:Biophys. J.
Volume:68
Issue / Number:5
Start Page:1687
End Page:1693
Review Status:Peer-review
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:We combined scanning force microscopy with patch-clamp techniques in the same experimental setup and obtained images of excised membrane patches spanning the tip of a glass pipette. These images indicate that cytoskeleton structures are still present in such membrane patches and form a strong connection between the membrane and the glass wall. This gives the membrane patch the appearance of a tent, stabilized by a scaffold of ropes. The lateral resolution of the images depends strongly on the observed structures and can reach values as low as 10 nm on the cytoskeleton elements of a (inside-out) patch. The observations suggest that measurements of membrane elasticity can be made, opening the way for further studies on mechanical properties of cell membranes.
External Publication Status:published
Document Type:Article
Communicated by:Wulf Kaiser
Affiliations:MPI für medizinische Forschung/Abteilung Zellphysiologie/
Identifiers:URI:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=... [Abstract]
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