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          Institute: MPI für medizinische Forschung     Collection: Abteilung Zellphysiologie     Display Documents



ID: 22480.0, MPI für medizinische Forschung / Abteilung Zellphysiologie
Local neurotrophic repression of gene transcripts encoding fetal AChRs at rat neuromuscular synapses
Translation of Title:Local neurotrophic repression of gene transcripts encoding fetal AChRs at rat neuromuscular synapses
Authors:Kues, Winfried; Brenner, Hans R.; Sakmann, Bert; Witzemann, Veit
Language:English
Date of Publication (YYYY-MM-DD):1995-08
Title of Journal:Journal of Cell Biology
Journal Abbrev.:J. Cell Biol.
Volume:130
Issue / Number:4
Start Page:949
End Page:957
Review Status:Peer-review
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:The spatio-temporal expression patterns of mRNA transcripts coding for acetylcholine receptor (AChR) subunits and myogenic factors were measured in denervated rat soleus muscle and in soleus muscle chronically paralyzed for up to 12 d by conduction block of the sciatic nerve by tetrodotoxin (TTX). In denervated muscle the AChR alpha-, beta- , gamma-, and delta-subunit mRNAs were elevated with highest expression levels in the former synaptic and the perisynaptic region and with lower levels in the extrasynaptic fiber segments. In muscle paralyzed by nerve conduction block the alpha-, beta-, gamma-, and delta-subunit mRNA levels increased only in extrasynaptic fiber segments. Surprisingly, in the synaptic region the gamma-subunit mRNA that specifies the fetal-type AChR, and alpha-, beta-, delta-subunit mRNAs were not elevated. The expression of the gene encoding the epsilon- subunit, which specifies the adult-type AChR, was always restricted to synaptic nuclei. The mRNA for the regulatory factor myogenin showed after denervation similar changes as the subunit transcripts of the fetal AChR. When the muscle was paralyzed by nerve conduction block the increase of myogenin transcripts was also less pronounced in synaptic regions compared to extrasynaptic fiber segments. The results suggest that in normal soleus muscle a neurotrophic signal from the nerve locally down-regulates the expression of fetal-type AChR channel in the synaptic and perisynaptic muscle membrane by inhibiting the expression of the gamma-subunit gene and that inhibition of the myogenin gene expression may contribute to this down-regulation.
External Publication Status:published
Document Type:Article
Communicated by:Wulf Kaiser
Affiliations:MPI für medizinische Forschung/Abteilung Zellphysiologie/
Identifiers:URI:http://www.jcb.org/cgi/content/abstract/130/4/949 [Abstract]
URI:http://www.jcb.org/cgi/reprint/130/4/949 [Fulltext PDF]
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