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          Institute: MPI für Entwicklungsbiologie     Collection: Abteilungsunabhängige Arbeitsgruppen     Display Documents



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ID: 225748.0, MPI für Entwicklungsbiologie / Abteilungsunabhängige Arbeitsgruppen
Abstract E-cadherin is essential for in vivo epidermal barrier function by regulating tight junctions.
Authors:Tunggal, J.A.; Helfrich, I.; Schmitz, A.; Schwarz, Heinz; Gunzel, D.; Fromm, M.; Kemler, R.; Krieg, T.; Niessen, C.M.
Language:English
Date of Publication (YYYY-MM-DD):2005-03-23
Title of Journal:EMBO Journal
Volume:24
Issue / Number:6
Start Page:1146
End Page:1156
Sequence Number of Article:15775979
Review Status:not specified
Audience:Not Specified
Abstract / Description:Cadherin adhesion molecules are key determinants of morphogenesis and tissue architecture. Nevertheless, the molecular mechanisms responsible for the morphogenetic contributions of cadherins remain poorly understood in vivo. Besides supporting cell-cell adhesion, cadherins can affect a wide range of cellular functions that include activation of cell signalling pathways, regulation of the cytoskeleton and control of cell polarity. To determine the role of E-cadherin in stratified epithelium of the epidermis, we have conditionally inactivated its gene in mice. Here we show that loss of E-cadherin in the epidermis in vivo results in perinatal death of mice due to the inability to retain a functional epidermal water barrier. Absence of E-cadherin leads to improper localization of key tight junctional proteins, resulting in permeable tight junctions and thus altered epidermal resistance. In addition, both Rac and activated atypical PKC, crucial for tight junction formation, are mislocalized. Surprisingly, our results indicate that E-cadherin is specifically required for tight junction, but not desmosome, formation and this appears to involve signalling rather than cell contact formation.
External Publication Status:published
Document Type:Article
Affiliations:MPI für Entwicklungsbiologie/Abteilungsunabhängige Arbeitsgruppen
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