Home News About Us Contact Contributors Disclaimer Privacy Policy Help FAQ

Home
Search
Quick Search
Advanced
Fulltext
Browse
Collections
Persons
My eDoc
Session History
Login
Name:
Password:
Documentation
Help
Support Wiki
Direct access to
document ID:


          Institute: MPI für medizinische Forschung     Collection: Forschungsgruppe Bioorganische Chemie     Display Documents



ID: 22607.0, MPI für medizinische Forschung / Forschungsgruppe Bioorganische Chemie
Evaluating atomic models of F-actin with an undecagold-tagged phalloidin derivative
Translation of Title:Evaluating atomic models of F-actin with an undecagold-tagged phalloidin derivative
Authors:Steinmetz, M. O.; Stoffler, D.; Müller, S. A.; Jahn, Werner; Wolpensinger, B.; Goldie, K. N.; Engel, A.; Faulstich, Heinz; Aebi, U.
Language:English
Date of Publication (YYYY-MM-DD):1998-02-13
Title of Journal:Journal of Molecular Biology
Journal Abbrev.:J. Mol. Biol.
Volume:276
Issue / Number:1
Start Page:1
End Page:6
Review Status:Peer-review
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:We have prepared an undecagold-tagged phalloidin derivative to determine this mushroom toxins binding site and orientation within the F-actin filament by scanning transmission electron microscopy (STEM) and 3-D helical reconstruction. Remarkably, when stoichiometrically bound to F-actin, the undecagold moiety of the derivative could be directly visualized by STEM along the two half-staggered long-pitch helical strands of single filaments. Most importantly, the structural data obtained when combined with various biochemical constraints enabled us to critically evaluate two distinct atomic models of the F-actin filament (i.e. the Holmes-Lorenz versus the Schutt-Lindberg model). Taken together, our data are in excellent agreement with the Holmes-Lorenz model.
External Publication Status:published
Document Type:Article
Communicated by:Wulf Kaiser
Affiliations:MPI für medizinische Forschung/Abteilung Biophysik/
MPI für medizinische Forschung/Forschungsgruppe Bioorganische Chemie/
Identifiers:URI:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=... [Abstract]
The scope and number of records on eDoc is subject to the collection policies defined by each institute - see "info" button in the collection browse view.