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          Institute: MPI für medizinische Forschung     Collection: Abteilung Molekulare Neurobiologie     Display Documents



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ID: 226485.0, MPI für medizinische Forschung / Abteilung Molekulare Neurobiologie
Lentivirus−based genetic manipulations of cortical neurons and their optical and electrophysiological monitoring in vivo
Translation of Title:Lentivirus−based genetic manipulations of cortical neurons and their optical and electrophysiological monitoring in vivo
Authors:Dittgen, Tanjew; Nimmerjahn, Axel; Komai, Shoji; Licznerski, Pawel; Waters, David Jack; Margrie, Troy W.; Helmchen, Fritjof; Denk, Winfried; Brecht, Michael; Osten, Pavel
Language:English
Date of Publication (YYYY-MM-DD):2004-12-28
Title of Journal:Proceedings of the National Academy of Sciences of the USA
Journal Abbrev.:Proc. Natl. Acad. Sci. U. S. A.
Volume:101
Issue / Number:52
Start Page:18206
End Page:18211
Review Status:Peer-review
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:It is becoming increasingly clear that single cortical neurons encode complex and behaviorally relevant signals, but efficient means to study gene functions in small networks and single neurons in vivo are still lacking. Here, we establish a method for genetic manipulation and subsequent phenotypic analysis of individual cortical neurons in vivo. First, lentiviral vectors are used for neuron−specific gene delivery from {alpha}−calcium/calmodulin−dependent protein kinase II or Synapsin I promoters, optionally in combination with gene knockdown by means of U6 promoter−driven expression of short−interfering RNAs. Second, the phenotypic analysis at the level of single cortical cells is carried out by using two−photon microscopy−based techniques: high−resolution two−photon time−lapse imaging is used to monitor structural dynamics of dendritic spines and axonal projections, whereas cellular response properties are analyzed electrophysiologically by two−photon microscopydirected whole−cell recordings. This approach is ideally suited for analysis of gene functions in individual neurons in the intact brain.
Last Change of the Resource (YYYY-MM-DD):--
External Publication Status:published
Document Type:Article
Version Comment:Automatic journal name synchronization
Communicated by:Wulf Kaiser
Affiliations:MPI für medizinische Forschung/Abteilung Zellphysiologie
MPI für medizinische Forschung/Abteilung Molekulare Neurobiologie
MPI für medizinische Forschung/Abteilung Biomedizinische Optik
Relations:Has References-URI:http%3A%2F%2Fwww.pnas.org%2Fcgi%2Fexternal_ref%3Faccess_num%3D14634645%26link_type%3DMED
Has References-URI:http%3A%2F%2Fwww.pnas.org%2Fcgi%2Fexternal_ref%3Faccess_num%3D12590264%26link_type%3DMED
Has References-URI:http%3A%2F%2Fwww.pnas.org%2Fcgi%2Fexternal_ref%3Faccess_num%3D11086982%26link_type%3DMED
Has References-URI:http%3A%2F%2Fwww.pnas.org%2Fcgi%2Fexternal_ref%3Faccess_num%3D10630186%26link_type%3DMED
Has References-URI:http%3A%2F%2Fwww.pnas.org%2Fcgi%2Fexternal_ref%3Faccess_num%3D11813241%26link_type%3DMED
Identifiers:DOI:10.1073%2Fpnas.0407976101
URI:http%3A%2F%2Fwww.pnas.org%2Fcgi%2Fcontent%2Fabstra...
URI:http%3A%2F%2Fwww.pnas.org%2Fcgi%2Fcontent%2Ffull%2...
URI:http%3A%2F%2Fwww.pnas.org%2Fcgi%2Freprint%2F101%2F...
LOCALID:6320
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