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          Institute: MPI für medizinische Forschung     Collection: Abteilung Molekulare Neurobiologie     Display Documents



ID: 22732.0, MPI für medizinische Forschung / Abteilung Molekulare Neurobiologie
Q/R site editing in kainate receptor GluR5 and GluR6 pre-mRNAs requires distant intronic sequences
Translation of Title:Q/R site editing in kainate receptor GluR5 and GluR6 pre-mRNAs requires distant intronic sequences
Authors:Herb, Anne; Higuchi, Miyoko; Sprengel, Rolf; Seeburg, Peter H.
Language:English
Date of Publication (YYYY-MM-DD):1996-03-05
Title of Journal:PNAS Online 1990 - Present
Journal Abbrev.:Proc. Natl. Acad. Sci. U. S. A.
Volume:93
Issue / Number:5
Start Page:1875
End Page:1880
Review Status:Peer-review
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:RNA editing by adenosine deamination in brain-expressed pre-mRNAs for glutamate receptor (GluR) subunits alters gene-specified codons for functionally critical positions, such as the channels Q/R site. We show by transcript analysis of minigenes transiently expressed in PC-12 cells that, in contrast to GluR-B pre-mRNA, where the two editing sites (Q/R and R/G) require base pairing with nearby intronic editing site complementary sequences (ECSs), editing in GluR5 and GluR6 pre-mRNAs recruits an ECS located as far as 1900 nucleotides distal to the Q/R site. The exon-intron duplex structure of the GluR5 and GluR6 pre-mRNAs appears to be a substrate of double-stranded RNA-specific adenosine deaminase. This enzyme when co-expressed in HEK 293 cells preferentially targets the adenosine of the Q/R site and of an unpaired position in the ECS which is highly edited in brain.
External Publication Status:published
Document Type:Article
Communicated by:Wulf Kaiser
Affiliations:MPI für medizinische Forschung/Abteilung Molekulare Neurobiologie/
Identifiers:URI:http://www.pnas.org/cgi/content/abstract/93/5/1875... [Abstract]
URI:http://www.pnas.org/cgi/reprint/93/5/1875?maxtosho... [Fulltext PDF]
ISSN:0027-8424/96/931875-6/0
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