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          Institute: MPI für medizinische Forschung     Collection: Abteilung Molekulare Neurobiologie     Display Documents



ID: 22746.0, MPI für medizinische Forschung / Abteilung Molekulare Neurobiologie
RED2, a brain specific member of the RNA specific adenosine deaminase family
Translation of Title:RED2, a brain specific member of the RNA specific adenosine deaminase family
Authors:Melcher, Thorsten; Maas, Stefan; Herb, Anne; Sprengel, Rolf; Higuchi, Miyoko; Seeburg, Peter H.
Language:English
Date of Publication (YYYY-MM-DD):1996-12-13
Title of Journal:Journal of Biological Chemistry
Journal Abbrev.:J. Biol. Chem. (JBCHA3)
Volume:271
Issue / Number:50
Start Page:31795
End Page:31798
Review Status:Peer-review
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:The mammalian RNA-specific adenosine deaminases DRADA/dsRAD (alias ADAR) and RED1 (alias ADARB1) have been implicated in the site-selective editing of brain-expressed pre-mRNAs for glutamate receptor subunits and of antigenomic RNA of hepatitis delta virus. These enzymes are expressed in many if not all tissues, predicting an as yet unappreciated significance for adenosine deamination-mediated recoding of gene transcripts in the mammalian organism. We now report the molecular cloning of cDNA for RED2 (alias ADARB2), a third member of the RNA-specific adenosine deaminase family in the rodent. RED2 is closely sequence-related to RED1 but appears to be expressed only in the brain, where expression is widespread reaching highest levels in olfactory bulb and thalamus. RED2 further differs from RED1 in having a 54-residue amino-terminal extension which includes an arginine-rich motif. Different from DRADA and RED1, recombinantly expressed RED2 did not deaminate adenosines in extended synthetic dsRNA or in GluR-B pre-mRNA. However, a chimera of RED1 and RED2 edited the GluR-B Q/R and R/G sites with moderate efficiency. Our data suggest that RED2 may edit brain-specific transcripts with distinct structural features.
External Publication Status:published
Document Type:Article
Communicated by:Wulf Kaiser
Affiliations:MPI für medizinische Forschung/Abteilung Molekulare Neurobiologie/
Identifiers:URI:http://www.jbc.org/cgi/content/full/271/50/31795 [Full text]
URI:http://www.jbc.org/cgi/content/abstract/271/50/317... [Abstract]
URI:http://www.jbc.org/cgi/reprint/271/50/31795 [Fulltext PDF]
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