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          Institute: MPI für medizinische Forschung     Collection: Forschungsgruppe Bioorganische Chemie     Display Documents

ID: 22846.0, MPI für medizinische Forschung / Forschungsgruppe Bioorganische Chemie
Involvement of ezrin/moesin in de novo actin assembly on phagosomal membranes
Translation of Title:Involvement of ezrin/moesin in de novo actin assembly on phagosomal membranes
Authors:Defacque, Hélène; Egeberg, Morten; Habermann, Anja; Diakonova, Maria; Roy, Christian; Mangeat, Paul; Voelter, Wolfgang; Marriott, Gerard; Pfannstiel, Jens; Faulstich, Heinz; Griffiths, Gareth
Date of Publication (YYYY-MM-DD):2000
Title of Journal:EMBO Journal
Journal Abbrev.:EMBO J.
Start Page:199
End Page:212
Review Status:Peer-review
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:The current study focuses on the molecular mechanisms responsible for actin assembly on a defined membrane surface: the phagosome. Mature phagosomes were surrounded by filamentous actin in vivo in two different cell types. Fluorescence microscopy was used to study in vitro actin nucleation/polymerization (assembly) on the surface of phagosomes isolated from J774 mouse macrophages. In order to prevent non-specific actin polymerization during the assay, fluorescent G-actin was mixed with thymosin beta4. The cytoplasmic side of phagosomes induced de novo assembly and barbed end growth of actin filaments. This activity varied cyclically with the maturation state of phagosomes, both in vivo and in vitro. Peripheral membrane proteins are crucial components of this actin assembly machinery, and we demonstrate a role for ezrin and/or moesin in this process. We propose that this actin assembly process facilitates phagosome/endosome aggregation prior to membrane fusion.
Free Keywords:actin assembly; ezrin-radixin-moesin family; latex beads; phagocytosis
External Publication Status:published
Document Type:Article
Communicated by:Wulf Kaiser
Affiliations:MPI für medizinische Forschung/Forschungsgruppe Bioorganische Chemie/
Identifiers:URI:http://emboj.oupjournals.org/cgi/content/full/19/2... [Full text]
URI:http://emboj.oupjournals.org/cgi/content/abstract/... [Abstract]
URI:http://emboj.oupjournals.org/cgi/reprint/19/2/199 [Fulltext PDF]
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