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          Institute: MPI für medizinische Forschung     Collection: Abteilung Biophysik     Display Documents



ID: 22944.0, MPI für medizinische Forschung / Abteilung Biophysik
A transcript encoding a nucleic acid-binding protein specifically expressed in maize seeds
Translation of Title:A transcript encoding a nucleic acid-binding protein specifically expressed in maize seeds
Authors:Heyl, A.; Muth, J.; Santandrea, Geraldina; OConnell, T.; Serna, Antonio; Thompson, R. D.
Language:English
Date of Publication (YYYY-MM-DD):2001
Title of Journal:Molecular Genetics and Genomics MGG
Journal Abbrev.:Mol. Genet. Genomics
Volume:266
Start Page:180
End Page:189
Review Status:Peer-review
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:A cDNA clone has been obtained for a low-abundance, seed-specific mRNA that encodes a polypeptide which defines a novel family of plant proteins with some similarities to the DnaJ class of molecular chaperones. The MEM1 (Maize Endosperm Motif binding protein) protein is capable of binding to the endosperm motif and activating transcription in the yeast one-hybrid system. Recombinant MEM1 was shown to bind in vitro to nucleic acids, with a preference for RNA over DNA. MEM1 is capable of forming homodimers, a property that is dependent on a domain close to the C-terminus of the protein. The protein is expressed in mid- to late-term endosperm cells. Subcellular fractionation and size fractionation under non-denaturing conditions indicate that the protein is present in the cytosol of endosperm cells. Possible roles of MEM1 in endosperm and protein body development are discussed.
Free Keywords:DNA; RNA; RNA-binding; DnaJ; Seed
External Publication Status:published
Document Type:Article
Communicated by:Wulf Kaiser
Affiliations:MPI für medizinische Forschung/Abteilung Biophysik/
Identifiers:URI:http://link.springer.de/link/service/journals/0043... [Full text]
URI:http://link.springer.de/link/service/journals/0043... [Abstract]
URI:http://link.springer.de/link/service/journals/0043... [Fulltext PDF]
DOI:10.1007/s004380100561
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