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          Institute: MPI für molekulare Genetik     Collection: Department of Developmental Genetics     Display Documents

ID: 229486.0, MPI für molekulare Genetik / Department of Developmental Genetics
A DNA microarray for fission yeast: minimal changes in global gene expression after temperature shift
Authors:Xue, Yongtao T.; Haas, Stefan; Brino, Laurento; Gusnanto, Arief; Riemers, Mark; Talibi, Driss; Vingron, Martin; Ekwall, Karl; Wright, Anthony P. H.
Date of Publication (YYYY-MM-DD):2004-01-01
Title of Journal:Yeast
Journal Abbrev.:Yeast
Issue / Number:1
Start Page:25
End Page:39
Copyright:© 2004 John Wiley & Sons, Ltd.
Review Status:not specified
Audience:Experts Only
Abstract / Description:Completion of the fission yeast genome sequence has opened up possibilities for post-genomic approaches. We have constructed a DNA microarray for genome-wide gene expression analysis in fission yeast. The microarray contains DNA fragments, PCR-amplified from a genomic DNA template, that represent > 99% of the 5000 or so annotated fission yeast genes, as well as a number of control sequences. The GenomePRIDE software used attempts to design similarly sized DNA fragments corresponding to gene regions within single exons, near the 3-end of genes that lack homology to other fission yeast genes. To validate the design and utility of the array, we studied expression changes after a 2 h temperature shift from 25 °C to 36 °C, conditions widely used when studying temperature-sensitive mutants. Obligingly, the vast majority of genes do not change more than two-fold, supporting the widely held view that temperature-shift experiments specifically reveal phenotypes associated with temperature-sensitive mutants. However, we did identify a small group of genes that showed a reproducible change in expression. Importantly, most of these corresponded to previously characterized heat-shock genes, whose expression has been reported to change after more extreme temperature shifts than those used here. We conclude that the DNA microarray represents a useful resource for fission yeast researchers as well as the broader yeast community, since it will facilitate comparison with the distantly related budding yeast, Saccharomyces cerevisiae. To maximize the utility of this resource, the array and its component parts are fully described in On-line Supplementary Information and are also available commercially.
Free Keywords:fission yeast • microarray • functional genomics • temperature shift • gene expression
Comment of the Author/Creator:Received: 15 June 2003; Accepted: 17 September 2003
Published Online: 19 Dec 2003
External Publication Status:published
Document Type:Article
Communicated by:Bernhard G. Herrmann
Affiliations:MPI für molekulare Genetik
External Affiliations:Natural Sciences Section, Södertörns University College, S-141 89 Huddinge, Sweden
Department of Biosciences, Karolinska Institute, Novum, S-141 57, Sweden
Eurogentec SA, Parc Scientifique du Sart Tilman, 4102 Seraing, Belgium
5Centre for Genomics and Bioinformatics, Karolinska Institute, S-171 77, Stockholm, Sweden
Department of Medical Epidemiology and Biostatistics, Karolinska Institute, S-171 77, Stockholm, Sweden
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