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          Institute: MPI für molekulare Genetik     Collection: Department of Computational Molecular Biology     Display Documents



ID: 229499.0, MPI für molekulare Genetik / Department of Computational Molecular Biology
Gene expression changes in the course of neural progenitor cell differentiation
Authors:Gurok, Ulf; Steinhoff, Christine; Lipkowitz, Bettina; Ropers, Hans-Hilger; Scharff, Constance; Nuber, Ulrike A.
Language:English
Date of Publication (YYYY-MM-DD):2004-06-30
Title of Journal:Journal of Neuroscience
Journal Abbrev.:J. Neurosci.
Volume:24
Issue / Number:26
Start Page:5982
End Page:6002
Copyright:Copyright © 2004 by Society for Neuroscience
Review Status:not specified
Audience:Experts Only
Abstract / Description:The molecular changes underlying neural progenitor differentiation are essentially unknown. We applied cDNA microarrays with 13,627 clones to measure dynamic gene expression changes during the in vitro differentiation of neural progenitor cells that were isolated from the subventricular zone of postnatal day 7 mice and grown in vitro as neurospheres. In two experimental series in which we withdrew epidermal growth factor and added the neurotrophins Neurotrophin-4 or BDNF, four time points were investigated: undifferentiated cells grown as neurospheres, and cells 24, 48, and 96 hr after differentiation. Expression changes of selected genes were confirmed by semiquantitative RT-PCR. Ten different groups of gene expression dynamics obtained by cluster analysis are described. To correlate selected gene expression changes to the localization of respective proteins, we performed immunostainings of cultured neurospheres and of brain sections from adult mice. Our results provide new insights into the genetic program of neural progenitor differentiation and give strong hints to as yet unknown cellular communications within the adult subventricular zone stem cell niche.
Free Keywords:development; differentiation; hybridization; ventricle; stem cell; microarray; subventricular zone; rostral migratory stream; neurosphere
External Publication Status:published
Document Type:Article
Communicated by:Martin Vingron
Affiliations:MPI für molekulare Genetik
External Affiliations:Freie Universitaet Berlin, Fachbereich Biologie, Chemie, Pharmazie, 14195 Berlin, Germany.
Identifiers:ISSN:0270-6474
DOI:10.1523/JNEUROSCI.0809-04.2004
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