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          Institute: MPI für medizinische Forschung     Collection: Abteilung Biophysik     Display Documents



ID: 22953.0, MPI für medizinische Forschung / Abteilung Biophysik
Single-molecule tracking of myosins with genetically engineered amplifier domains
Translation of Title:Single-molecule tracking of myosins with genetically engineered amplifier domains
Authors:Ruff, Christine; Furch, Marcus; Brenner, Bernhard; Manstein, Dietmar J.; Meyhöfer, Edgar
Language:English
Date of Publication (YYYY-MM-DD):2001-03
Title of Journal:Nature Structural Biology
Journal Abbrev.:Nat. Struct. Biol.
Volume:8
Issue / Number:3
Start Page:226
End Page:229
Review Status:Peer-review
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:We combined protein engineering and single molecule measurements to directly record the step size of a series of myosin constructs with shortened and elongated artificial neck domains. Our results show that the step size has a clear linear dependence on the length of the neck domain and we also established that mechanical amplification in the myosin motor is based on a rotation of the neck domain relative to the actin-bound head. For all our constructs, including those with artificial necks, the magnitude of the neck rotation concurrent with the displacement step was 30°. The engineered change in the step size of myosin marks a significant advance in our ability to selectively modify the functional properties of molecular motors.
External Publication Status:published
Document Type:Article
Communicated by:Wulf Kaiser
Affiliations:MPI für medizinische Forschung/Abteilung Biophysik/
Identifiers:URI:http://www.nature.com/cgi-taf/DynaPage.taf?file=/n... [Full text]
URI:http://www.nature.com/cgi-taf/DynaPage.taf?file=/n... [Abstract]
URI:http://www.nature.com/cgi-taf/DynaPage.taf?file=/n... [Fulltext PDF]
DOI:10.1038/84962
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