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          Institute: MPI für medizinische Forschung     Collection: Abteilung Biophysik     Display Documents

ID: 22974.0, MPI für medizinische Forschung / Abteilung Biophysik
Topology of the Polypeptide Chain in the Complex of Agglutinin from Castor Bean Seeds with -D-Galactose in the Crystalline State
Translation of Title:Topology of the Polypeptide Chain in the Complex of Agglutinin from Castor Bean Seeds with -D-Galactose in the Crystalline State
Authors:Konareva, N. V.; Gabdulkhakov, A. G.; Eschenburg, Susanne; Stoeva, Stanka; Popov, A. N.; Krauspenhaar, R.; Andrianova, M. E.; Savochkina, Y.; Agapov, I. I.; Tonevitskiframe, A. G.; Kornev, A. N.; Kornilov, V. V.; Zaitsev, V. N.; Voelter, W.; Betzel, Christian; Nikonow, S. V.; Vainshtein, B. K.; Mikhailov, Alexander S.
Date of Publication (YYYY-MM-DD):2001-04-25
Title of Journal:Crystallography Reports
Journal Abbrev.:Crystallography Reports
Issue / Number:5
Start Page:792
End Page:800
Review Status:Peer-review
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:The three-dimensional structure of the complex of agglutinin from Ricinus communis with frame3Dframe4galactose was established and refined at 2.5 ? resolution by X-ray structure analysis. Biocrystals were obtained using dialysis through a semipermeable membrane. X-ray intensity data (Rmerge = 4.6%) were collected from one crystal at 100 K using synchrotron radiation at the DESY outstation [European Molecular Biology Laboratory (EMBL), Hamburg, Germany]. The initial phases were calculated by the molecular replacement method. The atoms of both protein and sugar molecules were localized. Unlike ricin, the ricinlike heterodimer RcA contains only one galactose-binding center in the region of the Asn46-Gly25-Trp37-Lys40 site in the first domain of the B subunit, whereas the second galactose-binding site of the B subunit is lost. One functionally important water molecule, which is bound to the residues Tyr123-Glu176-Arg179-Glu207, wasrevealed in the region of the active center in the A subunit.
External Publication Status:published
Document Type:Article
Communicated by:Wulf Kaiser
Affiliations:MPI für medizinische Forschung/Abteilung Biophysik/
Identifiers:URI:http://www.maik.rssi.ru/cgi-bin/search.pl?type=abs... [Abstract]
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