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          Institute: MPI für molekulare Pflanzenphysiologie     Collection: Publikationen Pflanzenphysiologie     Display Documents



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ID: 230000.0, MPI für molekulare Pflanzenphysiologie / Publikationen Pflanzenphysiologie
The Arabidopsis SKU6/SPIRAL1 gene encodes a plus end-localized microtubule-interacting protein involved in directional cell expansion
Authors:Sedbrook, J. C.; Ehrhardt, D. W.; Fisher, S. E.; Scheible, W. R.; Somerville, C. R.
Language:English
Date of Publication (YYYY-MM-DD):2004-06
Title of Journal:Plant Cell
Journal Abbrev.:Plant Cell
Volume:16
Issue / Number:6
Start Page:1506
End Page:1520
Review Status:not specified
Audience:Not Specified
Abstract / Description:The sku6-1 mutant of Arabidopsis thaliana exhibits altered patterns of root and organ growth. sku6 roots, etiolated hypocotyls, and leaf petioles exhibit right-handed axial twisting, and root growth on inclined agar media is strongly right skewed. The touch-dependent sku6 root skewing phenotype is suppressed by the antimicrotubule drugs propyzamide and oryzalin, and right skewing is exacerbated by cold treatment. Cloning revealed that sku6-1 is allelic to spiral1-1 (spr1-1). However, modifiers in the Columbia (Col) and Landsberg erecta (Ler) ecotype backgrounds mask noncomplementation in sku6-1 (Col)/spr1-1 (Ler) F1 plants. The SPR1 gene encodes a plant-specific 12-kD protein that is ubiquitously expressed and belongs to a six-member gene family in Arabidopsis. An SPR1:green fluorescent protein (GFP) fusion expressed in transgenic seedlings localized to microtubules within the cortical array, preprophase band, phragmoplast, and mitotic spindle. SPR1:GFP was concentrated at the growing ends of cortical microtubules and was dependent on polymer growth state; the microtubule-related fluorescence dissipated upon polymer shortening. The protein has a repeated motif at both ends, separated by a predicted rod-like domain, suggesting that it may act as an intermolecular linker. These observations suggest that SPR1 is involved in microtubule polymerization dynamics and/or guidance, which in turn influences touch-induced directional cell expansion and axial twisting.
Free Keywords:root-growth
; in-vivo
; dynamic instability
; thaliana
; eb1
; gravitropism
; cytoskeleton
; alignment
; gravity
; binding
External Publication Status:published
Document Type:Article
Communicated by:N. N.
Affiliations:MPI für molekulare Pflanzenphysiologie/Metabolische Netzwerke/AG Scheible
External Affiliations:Illinois State Univ, Dept Biol Sci, Normal, IL 61790 USA
Illinois State Univ, Dept Biol Sci, Normal, IL 61790 USA
Carnegie Inst, Stanford, CA 94305 USA
Max Planck Inst Mol Plant Physiol, D-14476 Golm, Germany
Stanford Univ, Dept Biol Sci, Stanford, CA 94305 USA
Identifiers:ISI:000221984700014 [ID No:1]
ISI:000221984700014 [ID No:2]
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