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          Institute: MPI für medizinische Forschung     Collection: Max-Planck-Forschungsgruppe Ionenkanalstruktur (Dean R. Madden)     Display Documents



ID: 23008.0, MPI für medizinische Forschung / Max-Planck-Forschungsgruppe Ionenkanalstruktur (Dean R. Madden)
Characterization of transmembrane domains 3, 4, and 5 of MalF by mutational analysis
Translation of Title:Characterization of transmembrane domains 3, 4, and 5 of MalF by mutational analysis
Authors:Steinke, Angelika; Grau, Sandra; Davidson, Amy; Hofmann, Eckhard; Ehrmann, Michael
Language:English
Date of Publication (YYYY-MM-DD):2001
Title of Journal:Journal of Bacteriology
Journal Abbrev.:Journal of Bacteriology
Volume:183
Issue / Number:1
Start Page:375
End Page:381
Review Status:Peer-review
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:MalF and MalG are the cytoplasmic membrane components of the binding protein-dependent ATP binding cassette maltose transporter in Escherichia coli. They are thought to form the transport channel and are thus of critical importance for the mechanism of transport. To study the contributions of individual transmembrane segments of MalF, we isolated 27 point mutations in membrane-spanning segments 3, 4, and 5. These data complement a previous study, which described the mutagenesis of membrane-spanning segments 6, 7, and 8. While most of the isolated mutations appear to cause assembly defects, L323Q in helix 5 could interfere more directly with substrate specificity. The phenotypes and locations of the mutations are consistent with a previously postulated structural model of MalF.
External Publication Status:published
Document Type:Article
Communicated by:Wulf Kaiser
Affiliations:MPI für medizinische Forschung/Nachwuchsgruppe Ion Channel Structure/
Identifiers:URI:http://jb.asm.org/cgi/content/full/183/1/375?maxto... [Full text]
URI:http://jb.asm.org/cgi/content/abstract/183/1/375?m... [Abstract]
URI:http://jb.asm.org/cgi/reprint/183/1/375?maxtoshow=... [Fulltext PDF]
DOI:10.1128/JB.183.1.375-381
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