Please note that eDoc will be permanently shut down in the first quarter of 2021!      Home News About Us Contact Contributors Disclaimer Privacy Policy Help FAQ

Home
Search
Quick Search
Advanced
Fulltext
Browse
Collections
Persons
My eDoc
Session History
Login
Name:
Password:
Documentation
Help
Support Wiki
Direct access to
document ID:


          Institute: MPI für molekulare Genetik     Collection: Department of Vertebrate Genomics     Display Documents



  history
ID: 230601.0, MPI für molekulare Genetik / Department of Vertebrate Genomics
An endoribonuclease-prepared siRNA screen in human cells identifies genes essential for cell division
Authors:Kittler, Ralf; Putz, Gabriele; Pelletier, Laurence; Poser, Ina; Heninger, Anne-Kristin; Drechsel, David; Fischer, Steffi; Konstantinova, Irena; Habermann, Bianca; Grabner, Hannes; Yaspo, Marie-Laure; Himmelbauer, Heinz; Korn, Bernd; Neugebauer, Karla; Pisabarro, Maria Teresa; Buchholz, Frank
Language:English
Date of Publication (YYYY-MM-DD):2004-12-23
Title of Journal:Nature
Volume:432
Issue / Number:7020
Start Page:1036
End Page:1040
Copyright:© 2004 Nature Publishing Group
Review Status:not specified
Audience:Experts Only
Abstract / Description:RNA interference (RNAi) is an evolutionarily conserved defence mechanism whereby genes are specifically silenced through degradation of messenger RNAs; this process is mediated by homologous double-stranded (ds)RNA molecules. In invertebrates, long dsRNAs have been used for genome-wide screens and have provided insights into gene functions. Because long dsRNA triggers a nonspecific interferon response in many vertebrates, short interfering (si)RNA or short hairpin (sh)RNAs must be used for these organisms to ensure specific gene silencing. Here we report the generation of a genome-scale library of endoribonuclease-prepared short interfering (esi)RNAs from a sequence-verified complementary DNA collection representing 15,497 human genes. We used 5,305 esiRNAs from this library to screen for genes required for cell division in HeLa cells. Using a primary high-throughput cell viability screen followed by a secondary high content videomicroscopy assay, we identified 37 genes required for cell division. These include several splicing factors for which knockdown generates mitotic spindle defects. In addition, a putative nuclear-export terminator was found to speed up cell proliferation and mitotic progression after knockdown. Thus, our study uncovers new aspects of cell division and establishes esiRNA as a versatile approach for genomic RNAi screens in mammalian cells.
External Publication Status:published
Document Type:Article
Communicated by:Hans Lehrach
Affiliations:MPI für molekulare Genetik
External Affiliations:Max Planck Institute for Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, D-01307 Dresden, Germany;
Scionics Computer Innovation, GmbH, Pfotenhauerstrasse 110, D-01307 Dresden, Germany;
RZPD-Ressourcenzentrum für Genomforschung, Im Neuenheimer Feld 506, D-69120 Heidelberg, Germany
Identifiers:ISSN:0028-0836
DOI:10.1038/nature03159
The scope and number of records on eDoc is subject to the collection policies defined by each institute - see "info" button in the collection browse view.