Home News About Us Contact Contributors Disclaimer Privacy Policy Help FAQ

Quick Search
My eDoc
Session History
Support Wiki
Direct access to
document ID:

          Institute: MPI für molekulare Genetik     Collection: Department of Vertebrate Genomics     Display Documents

ID: 230625.0, MPI für molekulare Genetik / Department of Vertebrate Genomics
Studies on the optimisation and application of
protein arrays
Authors:Angenendt, Philipp
Date of Approval (YYYY-MM-DD):2004-07-30
Name of University:Freie Universität
Place of University:Berlin
Audience:Experts Only
Abstract / Description:The sequencing of the human genome and other ongoing sequencing projects have accelerated the pace of gene discovery and caused the identification of thousands of new genes. However, it also entails realisation that the genome alone could not provide enough information to understand the complex cellular network on the molecular level. Although genetic information provides us with the sequence of each protein, it is currently not possible to entirely deduce its localisation, structure, modifications, interactions, activities, and, ultimately, their function from it sequence. This lack of information becomes especially
obvious upon observation of a relatively closely linked relationship, the stoichiometry between RNA transcripts and their corresponding protein abundances. Although gene-protein dynamics were analysed for several tissues (1, 2), there is still no reliable correlation between gene activity and protein abundance. Besides this, protein abundances and their entirety, the proteome, are highly dynamic and therefore require tools that are amenable for describing several variables simultaneously. Up to today two-dimensional (2D) gel electrophoresis for protein separation, followed by mass spectrometry (MS) and database searches for protein identification, are the only real high-throughput techniques for the complex description of a
proteome. They are especially important in the classical proteome analysis, which focuses on studying complete proteomes, e.g. from two differentially treated cell lines, and the corresponding identification of single proteins.
Document Type:PhD-Thesis
Communicated by:Hans Lehrach
Affiliations:MPI für molekulare Genetik
External Affiliations:Fachbereich Biologie, Chemie, Pharmazie der Freien Universität Berlin
Full Text:
Sorry, no privileges
The scope and number of records on eDoc is subject to the collection policies defined by each institute - see "info" button in the collection browse view.