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          Institute: MPI für medizinische Forschung     Collection: Abteilung Biophysik     Display Documents



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ID: 23114.0, MPI für medizinische Forschung / Abteilung Biophysik
Multivariate statistical analysis (MSA) improves azimuthal alignment along the axis of helical objects and results in higher resolution for reconstruction using a single particle approach
Translation of Title:Multivariate statistical analysis (MSA) improves azimuthal alignment along the axis of helical objects and results in higher resolution for reconstruction using a single particle approach
Authors:Schröder, Rasmus R.; Angert, Isabel; Frank, Joachim; Holmes, Kenneth C.
Language:English
Date of Publication (YYYY-MM-DD):2002
Title of Journal:Biophysical Journal (Annual Meeting Abstracts)
Journal Abbrev.:Biophys. J. (Annual Meeting Abstracts)
Volume:82
Issue / Number:1
Start Page:491
End Page:491
Review Status:Peer-review
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:To obtain high structural resolution for reconstruction of helical objects by single particle methods a highly accurate alignment along the helical axis is necessary. In case of the actin-myosin-complex a typical misalignment on the order of the helical symmetry (±12.7°) limits resolution to 1-2.5nm depending on radius. Applying MSA to conventionally aligned 2D-projections different projection angles are distinguished. If a low resolution reconstructed density is available 2D-projections of that density along different azimuthal angles can be used to define a multi-dimensional factor space. MSA then defines a 1D-sub-space including its scale for the projection angle. Matching model data to experimental data for signal strength, resolution, and CTF, these experimental data can then directly be mapped into the given factor space. The experimental data shows a close distribution around the sub-space. An azimuthal angle can be assigned by determining the smallest distance to the sub-space. In a first application using the actin-myosin- complex it is possible to reduce the error in azimuthal alignment to less than ±3°. The effect on the final reconstruction and further refinement strategies are discussed.
External Publication Status:published
Document Type:Article
Communicated by:Wulf Kaiser
Affiliations:MPI für medizinische Forschung/Abteilung Biophysik
Identifiers:URI:http://meeting.biophysj.org/cgi/reprint/82/1/491/a... [Fulltext PDF]
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