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          Institute: MPI für medizinische Forschung     Collection: Abteilung Biophysik     Display Documents



  history
ID: 23120.0, MPI für medizinische Forschung / Abteilung Biophysik
Mutations in the relay loop region result in dominant-negative inhibition of myosin II function in Dictyostelium
Translation of Title:Mutations in the relay loop region result in dominant-negative inhibition of myosin II function in Dictyostelium
Authors:Tsiavaliaris, Georgios; Fujita-Becker, Setsuko; Batra, Renu; Levitsky, Dmitrii I.; Kull, F. Jon; Geeves, Michael A.; Manstein, Dietmar J.
Language:English
Date of Publication (YYYY-MM-DD):2002-11
Title of Journal:EMBO Reports
Journal Abbrev.:EMBO reports
Volume:3
Issue / Number:11
Start Page:1099
End Page:1105
Review Status:Peer-review
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:Dominant-negative inhibition is a powerful genetic tool for the characterization of gene function in vivo, based on the specific impairment of a gene product by the coexpression of a mutant version of the same gene product. We describe the detailed characterization of two myosin constructs containing either point mutations F487A or F506G in the relay region. Dictyostelium cells transformed with F487A or F506G myosin are unable to undergo processes that require myosin II function, including fruiting-body formation, normal cytokinesis and growth in suspension. Our results show that the dominant-negative inhibition of myosin function is caused by disruption of the communication between active site and lever arm, which blocks motor activity completely, and perturbation of the communication between active site and actin-binding site, leading to an 100-fold increase in the mutants' affinity for actin in the presence of ATP.
External Publication Status:published
Document Type:Article
Communicated by:Wulf Kaiser
Affiliations:MPI für medizinische Forschung/Abteilung Biophysik
Identifiers:URI:http://www.nature.com/cgi-taf/DynaPage.taf?file=/e... [Fulltext HTML]
URI:http://www.nature.com/cgi-taf/DynaPage.taf?file=/e... [Abstract]
URI:http://www.nature.com/cgi-taf/DynaPage.taf?file=/e... [Fulltext PDF]
DOI:10.1093/embo-reports/kvf214
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