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          Institute: MPI für medizinische Forschung     Collection: Abteilung Zellphysiologie     Display Documents

ID: 23194.0, MPI für medizinische Forschung / Abteilung Zellphysiologie
Small N-terminal deletion by splicing in cerebellar a6 subunit abolishes GABAA receptor function
Translation of Title:Small N-terminal deletion by splicing in cerebellar a6 subunit abolishes GABAA receptor function
Authors:Korpi, Esa R.; Kuner, Thomas; Kristo, P.; Köhler, Martin; Herb, Anne; Lüddens, H.; Seeburg, Peter H.
Date of Publication (YYYY-MM-DD):1994-09
Title of Journal:Journal of Neurochemistry
Journal Abbrev.:J. Neurochem.
Issue / Number:3
Start Page:1167
End Page:1170
Review Status:Peer-review
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:Sequence variation was found in cDNA coding for the extracellular domain of the rat gamma-aminobutyric acid type A (GABAA) receptor alpha 6 subunit. About 20% of polymerase chain reaction (PCR)-amplified alpha 6 cDNA prepared from rat cerebellar mRNA lacked nucleotides 226-255 as estimated by counting single-stranded phage plaques hybridized specifically to the short (alpha 6S) and long (wild-type) forms of the alpha 6 mRNA. Genomic PCR revealed an intron located upstream of the 30-nucleotide sequence. Both splice forms were detected in the cerebellum by in situ hybridization. Recombinant receptors, resulting from coexpression of the alpha 6S subunit with the GABAA receptor beta 2 and gamma 2 subunits in human embryonic kidney 293 cells, were inactive at binding [3H]muscimol and [3H]Ro 15-4513. In agreement, injection of complementary RNAs encoding the same subunits into Xenopus oocytes produced only weak GABA-induced currents, indistinguishable from those produced by beta 2 gamma 2 receptors. Therefore, the 10 amino acids encoded by the 30-nucleotide fragment may be essential for the correct assembly or folding of the alpha 6 subunit-containing receptors.
External Publication Status:published
Document Type:Article
Communicated by:Wulf Kaiser
Affiliations:MPI für medizinische Forschung/Abteilung Zellphysiologie/
MPI für medizinische Forschung/Abteilung Molekulare Neurobiologie/
Identifiers:URI:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=... [Abstract]
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