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          Institute: MPI für medizinische Forschung     Collection: Abteilung Zellphysiologie     Display Documents



ID: 23204.0, MPI für medizinische Forschung / Abteilung Zellphysiologie
Editing of α-amino-3-hydroxy-5-methylisoxazole-4-propionic acid receptor GluR-B pre-mRNA in vitro reveals site-selective adenosine to inosine conversion
Translation of Title:Editing of α-amino-3-hydroxy-5-methylisoxazole-4-propionic acid receptor GluR-B pre-mRNA in vitro reveals site-selective adenosine to inosine conversion
Authors:Melcher, Thorsten; Maas, Stefan; Higuchi, Miyoko; Keller, Walter; Seeburg, Peter H.; Major, G.; Larkman, A. U.; Jonas, Peter; Sakmann, Bert; Jack, J. J. B.
Language:English
Date of Publication (YYYY-MM-DD):1995-04-14
Title of Journal:Journal of Biological Chemistry
Journal Abbrev.:J. Biol. Chem. (JBCHA3)
Volume:270
Issue / Number:15
Start Page:8566
End Page:8570
Review Status:Peer-review
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:In neurons of the mammalian brain primary transcripts of genes encoding subunits of glutamate receptor channels can undergo RNA editing, leading to altered properties of the transmitter-activated channel. Editing of these transcripts is a nuclear process that targets specific adenosines and requires a double-stranded RNA structure configured from complementary exonic and intronic sequences. We show here that the two independent editing sites in -amino-3-hydroxy-5-methylisoxazole-4-propionic acid receptor GluR-B pre-mRNA are edited with positional accuracy by nuclear extract from HeLa cells. Nucleotide analysis by thin layer chromatography of the edited RNA sequences revealed selective adenosine to inosine conversion, most likely reflecting the participation of double-stranded RNA adenosine deaminase. Our results predict the presence of inosine-containing codons in other mammalian mRNAs.
External Publication Status:published
Document Type:Article
Communicated by:Wulf Kaiser
Affiliations:MPI für medizinische Forschung/Abteilung Zellphysiologie/
MPI für medizinische Forschung/Abteilung Molekulare Neurobiologie/
Identifiers:URI:http://www.jbc.org/cgi/content/full/270/15/8566 [Full text]
URI:http://www.jbc.org/cgi/content/abstract/270/15/856... [Abstract]
URI:http://www.jbc.org/cgi/reprint/270/15/8566 [Fulltext PDF]
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