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          Institute: MPI für biophysikalische Chemie     Collection: Molekulare Biologie (Dr. Thomas M. Jovin)     Display Documents



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ID: 233611.0, MPI für biophysikalische Chemie / Molekulare Biologie (Dr. Thomas M. Jovin)
Imaging the electrocyte of Torpedo marmorata by scanning force microscopy
Authors:Pietrasanta, L. I.; Schaper, A.; Fox, G. Q.; Barrantes, F. J.; Jovin, T. M.
Language:English
Date of Publication (YYYY-MM-DD):1996
Title of Journal:Scanning Microscopy
Volume:10
Start Page:963
End Page:974
Copyright:Jahrbuch 1999, Copyright MPG 1999
Review Status:Peer-review
Audience:Not Specified
Intended Educational Use:No
Abstract / Description:Scanning force microscopy (SFM) and scanning electron microscopy (SEM) were used to examine the tissue structure of the electric organ of Torpedo marmorata in air and in liquid after applying fracturing and cryosectioning techniques and chemical fixation. The electric organ is organized in columns of stacked electrocytes, arranged in a honeycomb pattern. The columns were cut along a plane normal to the cell stack and thin sections were transferred to polylysine coated glass cover slips. The polarity of the electrocytes was made apparent by immunofluorescence microscopy directed to different domains of the acetylcholine receptor (AChR), thus revealing the innervated face of the cell. SFM and SEM both showed cell surfaces to be overlaid by a network of collagen fibers by their characteristic banding pattern with ~64 nm periodicity and ~2.5 nm corrugation amplitude. In liquid, significantly lower structural resolution was achieved by SFM, probably due to sample elasticity.
Comment of the Author/Creator:this issue was really published in 1998
Last Change of the Resource (YYYY-MM-DD):2005-08-15
External Publication Status:published
Document Type:Article
Communicated by:N. N.
Affiliations:MPI für biophysikalische Chemie/Abt. Thomas Jovin / 060
External Affiliations:Instituto de Investigaciones Bioquimicas (INIBIBB), Bahia Blanca, Argentina
Identifiers:LOCALID:636
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