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          Institute: MPI für Herz- und Lungenforschung (W. G. Kerckhoff Institut)     Collection: Publikationen des W. G. Kerckhoff-Instituts     Display Documents

ID: 233640.0, MPI für Herz- und Lungenforschung (W. G. Kerckhoff Institut) / Publikationen des W. G. Kerckhoff-Instituts
Early cytoskeletal rearrangement during dendritic cell maturation enhances synapse formation and Ca(2+) signaling in CD8(+) T cells
Authors:Averbeck, M.; Braun, T.; Pfeifer, G.; Sleeman, J.; Dudda, J.; Martin, S. F.; Kremer, B.; Aktories, K.; Simon, J. C.; Termeer, C.
Date of Publication (YYYY-MM-DD):2004-10
Journal Abbrev.:Eur J Immunol
Issue / Number:10
Start Page:2708
End Page:2719
Audience:Not Specified
Abstract / Description:The interplay between dendritic cells (DC) and T cells is a dynamic process critically depending on DC maturation. Ca(2+) influx is one of the initial events occurring during DC/T cell contacts. To determine how DC maturation influences DC/T cell contacts, time-lapse video microscopy was established using TCR-transgenic CD8(+) T cells from P14 mice. DC maturation shifted DC/T cell contacts from short-lived interactions with transient Ca(2+) influx in T cells to long-lasting interactions and sustained Ca(2+) influx of 30 min and more. Follow-up of DC/T cell interactions after 2 h using confocal microscopy revealed that long-lasting Ca(2+) responses in T cells were preferentially associated with the formation of an immunological synapse involving CD54 and H2-K(b) at the DC/T cell interface. Such synapse formation preceded MHC or B7 up-regulation, since DC developed into potent Ca(2+) stimulators 7 h after initiation of maturation. Instead, the enhanced capacity of 7 h-matured DC to induce sustained Ca(2+) responses in CD8(+) T cells is critically dependent on the polarization and rearrangement of the cytoskeleton, as shown by Clostridium difficile toxin B inhibitor experiments. These data indicate that already very early after receiving a maturation stimulus, DC display enhanced cytoskeletal activity resulting in the rapid formation of immunological synapses and effective CD8(+) T cell stimulation.
Free Keywords:Animals ; Antigen Presentation/immunology ; CD8-Positive T-Lymphocytes/*immunology/metabolism ; Calcium/*metabolism ; Cell Communication/immunology ; Cell Movement ; Cell Polarity ; Cytoskeleton/*physiology ; Dendritic Cells/*immunology/metabolism ; Flow Cytometry ; Histocompatibility Antigens Class II ; Intercellular Adhesion Molecule-1/immunology ; Lymphocyte Activation/immunology ; Mice ; Mice, Transgenic ; Microscopy, Confocal ; Microscopy, Video ; Receptors, Antigen, T-Cell/genetics ; Research Support, Non-U.S. Gov't ; Signal Transduction/*immunology
External Publication Status:published
Document Type:Article
Communicated by:N. N.
Affiliations:MPI für physiologische und klinische Forschung
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