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          Institute: MPI für biophysikalische Chemie     Collection: Molekulare Biologie (Dr. Thomas M. Jovin)     Display Documents



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ID: 239873.0, MPI für biophysikalische Chemie / Molekulare Biologie (Dr. Thomas M. Jovin)
Superresolution by localization of quantum dots using blinking statistics
Authors:Lidke, K. A.; Rieger, B.; Jovin, T. M.; Heintzmann, R.
Language:English
Date of Publication (YYYY-MM-DD):2005-09-05
Title of Journal:Optics Express
Volume:13
Issue / Number:18
Start Page:7052
End Page:7062
Review Status:Peer-review
Audience:Not Specified
Intended Educational Use:No
Abstract / Description:In microscopy, single fluorescence point sources can be localized with a precision several times greater than the resolution limit of the microscope. We show that the intermittent fluorescence or ’blinking’ of quantum dots can analyzed by an Independent Component Analysis so as to identify the light emitted by each individual nanoparticle, localize it precisely, and thereby resolve groups of closely spaced (< λ /30) quantum dots. Both simulated and experimental data demonstrate that this technique is superior to localization based on Maximum Likelihood Estimation under the assumption of point emitters. This technique has general application to any emitter with non-Gaussian temporal intensity distribution, including triplet state blinking. When applied to the labeling of structures, a high resolution ”image” consisting of individually localized points may be reconstructed leading to the term ”Pointillism”.
Free Keywords:Superresolution; Fluorescence microscopy; Image reconstruction–restoration; Pattern Recognition; Resolution
Last Change of the Resource (YYYY-MM-DD):2005-09-07
External Publication Status:published
Document Type:Article
Communicated by:N. N.
Affiliations:MPI für biophysikalische Chemie/Abt. Thomas Jovin / 060
External Affiliations:King’s College London, Randall Division of Cell and Molecular Biophysics, New Hunt’s House, Guy’s Campus, London SE1 1UL
Identifiers:URL:http://www.opticsinfobase.org/oe/viewmedia.cfm?uri...
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