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          Institute: MPI für Dynamik komplexer technischer Systeme     Collection: Bioprocess Engineering     Display Documents



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ID: 245286.0, MPI für Dynamik komplexer technischer Systeme / Bioprocess Engineering
Serum-free influenza vaccine production with MDCK cells in wave-bioreactor and 5L-stirred tank bioreactor
Authors:Genzel, Y.; Fischer, M.; Olmer, R. M.; Schäfer, B.; Best, C.; König, S.; Hundt, B.; Reichl, U.
Language:English
Publisher:Springer
Place of Publication:Dordrecht, The Netherlands
Date of Publication (YYYY-MM-DD):2007
Title of Proceedings:Cell Technology for Cell Products : Proceedings of the 19th ESACT Meeting
Start Page:563
End Page:565
Name of Conference/Meeting:19th ESACT meeting
Place of Conference/Meeting:Harrogate, UK
(Start) Date of Conference/Meeting
 (YYYY-MM-DD):
2005-06-05
End Date of Conference/Meeting 
 (YYYY-MM-DD):
2005-06-08
Review Status:not specified
Audience:Experts Only
Abstract / Description:A serum-free process for influenza virus vaccine production (equine and human) in roller bottles and microcarrier systems in 5L-stirred tank and 2L-wave bioreactor (Cytodex 1) is described. MDCK cells were adapted from growth in serum containing GMEM medium to serum-free Ex-Cell MDCK medium. Virus titers of 2.0-2.9 log HA units/ 100 µL were obtained. Omission of the medium exchange before infection has clearly simplified the process.
External Publication Status:published
Document Type:Conference-Paper
Communicated by:Udo Reichl
Affiliations:MPI für Dynamik komplexer technischer Systeme/Bioprocess Engineering
External Affiliations:Lehrstuhl für Bioverfahrenstechnik, Universität Stuttgart, Stuttgart, Germany.
Lehrstuhl für Zellkulturtechnik, Universität Bielefeld, Bielefeld, Germany.
Lehrstuhl für Bioprozesstechnik, Otto-von-Guericke-Universität Magdeburg, Magdeburg, Germany.
Identifiers:URL:http://dx.doi.org/10.1007/978-1-4020-5476-1_97
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