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          Institute: MPI für biophysikalische Chemie     Collection: Molekulare Biologie (Dr. Thomas M. Jovin)     Display Documents



  history
ID: 245856.0, MPI für biophysikalische Chemie / Molekulare Biologie (Dr. Thomas M. Jovin)
Adaptation of nanoarrays for the study of α-synuclein aggregation - preliminary results
Authors:Dietrich, H. R. C.; van den Doel, R. L.; Hoyer, W.; van Oel, W.; Lung, G. L.; Garini, Y.; Jovin, T. M.; Young, I. T.
Language:English
Place of Publication:Bellingham, Wash.
Publisher:Society of Photo Optical Instrumentation Engineers
Date of Publication (YYYY-MM-DD):2005-03
Title of Book:Imaging, manipulation, and analysis of biomolecules and cells: fundamentals and applications III
Start Page:395
End Page:402
Sequence Number of Inbook:DOI: 10.1117/12.587493
Full Name of Book-Editor(s):Nicolau, D.V.
Title of Series:SPIE Proceedings
Volume:5699
Review Status:Peer-review
Audience:Not Specified
Intended Educational Use:No
Abstract / Description:In previous publications we have shown that we can perform enzymatic reactions in nanoarrays by means of a microarray-reader based on a conventional microscope. In this publication we report on a modification of this system in order to monitor the aggregation kinetics of the natively unfolded protein α-synuclein. We describe the motivation for this development, the problems associated with the miniaturization of the aggregation assay, and the validation of our modifications.
Free Keywords:Nanoarrays; High Throughput Screening; Evaporation Control; α-Synuclein; Thioflavin T; Quantitative Fluorescence Microscopy
External Publication Status:published
Document Type:InBook
Communicated by:N. N.
Affiliations:MPI für biophysikalische Chemie/Abt. Thomas Jovin / 060
External Affiliations:Department of Imaging Science and Technology, Faculty of Applied Sciences, Delft University of Technology, Lorentzweg 1, 2628 CJ Delft, The Netherlands
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