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          Institute: MPI für molekulare Genetik     Collection: Ribosome Research Groups     Display Documents

ID: 24722.0, MPI für molekulare Genetik / Ribosome Research Groups
Cryo-EM reveals an active role for aminoacyl-tRNA in the accommodation process
Authors:Valle, Mikel; Sengupta, Jayati; Swami, Neil K.; Grassucci, Robert A.; Burkhardt, Nils; Nierhaus, Knud H.; Agrawal, Rajendra K.; Frank, Joachim
Date of Publication (YYYY-MM-DD):2002-07
Title of Journal:EMBO Journal
Issue / Number:13
Start Page:3557
End Page:3567
Review Status:not specified
Audience:Experts Only
Abstract / Description:During the elongation cycle of protein biosynthesis, the specific amino acid coded for by the mRNA is delivered by a complex that is comprised of the cognate aminoacyl-tRNA, elongation factor Tu and GTP. As this ternary complex binds to the ribosome, the anticodon end of the tRNA reaches the decoding center in the 30S subunit. Here we present the cryo- electron microscopy (EM) study of an Escherichia coli 70S ribosome-bound ternary complex stalled with an antibiotic, kirromycin. In the cryo-EM map the anticodon arm of the tRNA presents a new conformation that appears to facilitate the initial codon–anticodon interaction. Furthermore, the elbow region of the tRNA is seen to contact the GTPase-associated center on the 50S subunit of the ribosome, suggesting an active role of the tRNA in the transmission of the signal prompting the GTP hydrolysis upon codon recognition.
Free Keywords:decoding/electron microscopy/elongation factor Tu/ribosome/tRNA
External Publication Status:published
Document Type:Article
Communicated by:Ribosome Group
Affiliations:MPI für molekulare Genetik
External Affiliations:Howard Hughes Medical Institute, Health Research, Inc., at the Wadsworth Center, New York State Department of Health, Empire State Plaza, Albany, NY 12201-0509
Columbia High School, 962 Luther Road, East Greenbush, NY 12061
Department of Biomedical Sciences, State University of New York at Albany, Empire State Plaza, Albany, NY 12201-0509, USA
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