Please note that eDoc will be permanently shut down in the first quarter of 2021!      Home News About Us Contact Contributors Disclaimer Privacy Policy Help FAQ

Quick Search
My eDoc
Session History
Support Wiki
Direct access to
document ID:

          Institute: MPI für molekulare Pflanzenphysiologie     Collection: Publikationen Pflanzenphysiologie     Display Documents

ID: 248399.0, MPI für molekulare Pflanzenphysiologie / Publikationen Pflanzenphysiologie
A proteomic fingerprint of dissolved organic carbon and of soil particles
Authors:Schulze, W. X.; Gleixner, G.; Kaiser, K.; Guggenberger, G.; Mann, M.; Schulze, E. D.
Date of Publication (YYYY-MM-DD):2005
Title of Journal:Oecologia
Issue / Number:3
Start Page:335
End Page:343
Review Status:not specified
Audience:Not Specified
Abstract / Description:Mass spectrometry-based proteomics was applied to analyze proteins isolated from dissolved organic matter (DOM). The focal question was to identify the type and biological origin of proteins in DOM, and to describe diversity of protein origin at the level of higher taxonomic units, as well as to detect extracellular enzymes possibly important in the carbon cycle. Identified proteins were classified according to their phylogenetic origin and metabolic function using the National Center for Biotechnology Information (NCBI) protein and taxonomy database. Seventy-eight percent of the proteins in DOM from the lake but less than 50% in forest soil DOM originated from bacteria. In a deciduous forest, the number of identified proteins decreased from 75 to 28 with increasing soil depth and decreasing total soil organic carbon content. The number of identified proteins and taxonomic groups was 50% higher in winter than in summer. In spruce forest, number of proteins and taxonomic groups decreased by 50% on a plot where trees had been girdled a year before and carbohydrate transport to roots was terminated. After girdling, proteins from four taxonomic groups remained as compared to nine taxonomic groups in healthy forest. Enzymes involved in degradation of organic matter were not identified in free soil DOM. However, cellulases and laccases were found among proteins extracted from soil particles, indicating that degradation of soil organic matter takes place in biofilms on particle surfaces. These results demonstrate a novel application of proteomics to obtain a "proteomic fingerprint'' of presence and activity of organisms in an ecosystem. [References: 37] 37
Free Keywords:Mass-spectrometry. Protein identification. Community structure.
; Matter. Pyrolysis. Ecosystem. Germany. Origin. Forest.
; Environment/Ecology in Current Contents(R)/Agricultural, Biology &
; Environmental Sciences.
; 2005 week 08
; Reprint available from: Schulze WX. Univ So Denmark, Dept Biochem & Mol
; Biol, CEBI, DK-5230 Odense, Denmark, .
External Publication Status:published
Document Type:Article
Communicated by:N. N.
Affiliations:MPI für molekulare Pflanzenphysiologie
The scope and number of records on eDoc is subject to the collection policies defined by each institute - see "info" button in the collection browse view.