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          Institute: MPI für molekulare Pflanzenphysiologie     Collection: Publikationen Pflanzenphysiologie     Display Documents



  history
ID: 248408.0, MPI für molekulare Pflanzenphysiologie / Publikationen Pflanzenphysiologie
An optical multifrequency phase-modulation method using microbeads for measuring intracellular oxygen concentrations in plants
Authors:Schmalzlin, E.; van Dongen, J. T.; Klimant, I.; Marmodee, B.; Steup, M.; Fisahn, J.; Geigenberger, P.; Lohmannsroben, H. G.
Language:English
Date of Publication (YYYY-MM-DD):2005-08
Title of Journal:Biophysical Journal
Journal Abbrev.:Biophys J
Volume:89
Issue / Number:2
Start Page:1339
End Page:1345
Review Status:not specified
Audience:Not Specified
Abstract / Description:A technique has been developed to measure absolute intracellular oxygen concentrations in green plants. Oxygen-sensitive phosphorescent microbeads were injected into the cells and an optical multifrequency phase-modulation technique was used to discriminate the sensor signal from the strong auto fluorescence of the plant tissue. The method was established using photosynthesis- competent cells of the giant algae Chara corallina L., and was validated by application to various cell types of other plant species.
Free Keywords:low internal oxygen
; growing potato-tubers
; fluorescent nanosensors
; storage metabolism
; live cells
; transport
; lifetime
; calcium
; growth
External Publication Status:published
Document Type:Article
Communicated by:N. N.
Affiliations:MPI für molekulare Pflanzenphysiologie
External Affiliations:Univ Potsdam, Inst Chem, Potsdam, Germany
Univ Potsdam, Inst Chem, Potsdam, Germany
Max Planck Inst Mol Plant Physiol, Golm, Germany
Graz Tech Univ, Inst Analyt Chem, A-8010 Graz, Austria
Univ Potsdam, Inst Biochem & Biol, Potsdam, Germany
Identifiers:ISI:000230822200055 [ID No:1]
ISI:000230822200055 [ID No:2]
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